CAJ | 학술논문

为研究水温对刺参(Apostichopus japonicus)消化酶及免疫酶活力的影响，本实验将体质量为8.0 g左右的刺参分别于10、14、18、22℃下养殖4周后，对其消化道中蛋白酶、淀粉酶、纤维素酶和脂肪酶活力，以及体腔液中溶菌酶和超氧化物歧化酶活力进行测定。研究结果显示，刺参消化道中蛋白酶含量最高，其酶活在10℃下最高，但随着温度的升高活力逐渐下降，在水温升至18℃以后酶活显著下降；淀粉酶活力在水温升至的18℃时无明显变化，但至22℃时活力显著下降；纤维素酶和脂肪酶活力较小，不同温度下其活力无显著差异。水温对刺参体腔液溶菌酶活性影响显著，在水温为18℃时达到最高值，升至22℃时其活力显著降低；超氧化物歧化酶活性在水温为18℃达到最低，但水温升至22℃其酶活与18℃相比又显著升高。
위연구수온대자삼(Apostichopus japonicus)소화매급면역매활력적영향，본실험장체질량위8.0 g좌우적자삼분별우10、14、18、22℃하양식4주후，대기소화도중단백매、정분매、섬유소매화지방매활력，이급체강액중용균매화초양화물기화매활력진행측정。연구결과현시，자삼소화도중단백매함량최고，기매활재10℃하최고，단수착온도적승고활력축점하강，재수온승지18℃이후매활현저하강；정분매활력재수온승지적18℃시무명현변화，단지22℃시활력현저하강；섬유소매화지방매활력교소，불동온도하기활력무현저차이。수온대자삼체강액용균매활성영향현저，재수온위18℃시체도최고치，승지22℃시기활력현저강저；초양화물기화매활성재수온위18℃체도최저，단수온승지22℃기매활여18℃상비우현저승고。
To investigate the effects of temperatures on the digestive enzymes and immune enzymes activities, sev-eral groups of sea cucumbers (Apostichopus japonicus) (approximately 8.0 g) were reared under different constant temperatures ranged from 10~22℃for four weeks. Then, the activities of protease, amylase, cellulase and lipase in the digestive tract and the lysozyme and superoxide dismutase (SOD) in coelomic fluid were determined. The re-sults showed that the protease was the most abundant enzyme in the digestive tract. The protease activity was high-est when the sea cucumbers were reared in 10℃, but it was significantly inhibited when the temperature was raised to 18℃(P<0.05). There was no significant difference in amylase activity between sea cucumbers groups reared in temperature below 18℃. However, the amylase activity was significant reduced in the sea cucumber group when temperature was increased to 22℃(P<0.05). The levels of cellulose and lipase activities were extremely low in all experimental groups. Moreover, it was found that the lysozyme activity was the highest when the sea cucumbers were reared in 18℃(P<0.05), but it was significantly decreased at 22℃(P<0.05). However, activity of SOD exhib-ited a different trend in sea cucumber groups. The SOD activity was gradually reduced as the temperature rose, but it was significantly increased when temperature rose up to 22℃(P<0.05).