CAJ | 학술논문

为了能够有效利用糙刺参内脏蛋白,以水解度为主要指标,对酶解蛋白酶进行了筛选,并系统研究了酶解温度、pH、加酶量和水解时间等单因素对水解度的影响。在此基础上,利用响应面中心组合设计实验,对酶解工艺进行了优化,得到以温度、pH、加酶量及水解时间为因子的二次方程,通过方差分析和验证性实验得出,此二次方程能较好反应海参内脏团蛋白水解度的变化规律,得到最佳水解条件为：温度52.66℃, pH 6.71,加酶量2.5%(质量分数),酶解时间10 h,预测最高水解度为49.58%。最佳酶解条件下所得多肽74%以上分子质量小于500 Da。利用DPPH法测定最佳条件酶解所得多肽的抗氧化活性,3 g/L的抗氧化率达到了24.8%,利用ABTS法测定最佳条件酶解所得多肽的抗氧化活性,3 g/L的抗氧化率达到了0.255μmol/g。为海参内脏蛋白的利用提供了参考。
위료능구유효이용조자삼내장단백,이수해도위주요지표,대매해단백매진행료사선,병계통연구료매해온도、pH、가매량화수해시간등단인소대수해도적영향。재차기출상,이용향응면중심조합설계실험,대매해공예진행료우화,득도이온도、pH、가매량급수해시간위인자적이차방정,통과방차분석화험증성실험득출,차이차방정능교호반응해삼내장단단백수해도적변화규률,득도최가수해조건위：온도52.66℃, pH 6.71,가매량2.5%(질량분수),매해시간10 h,예측최고수해도위49.58%。최가매해조건하소득다태74%이상분자질량소우500 Da。이용DPPH법측정최가조건매해소득다태적항양화활성,3 g/L적항양화솔체도료24.8%,이용ABTS법측정최가조건매해소득다태적항양화활성,3 g/L적항양화솔체도료0.255μmol/g。위해삼내장단백적이용제공료삼고。
In order to increase the utilization value of sea cucumber (Stichopus horrens) viscera as a potential source of peptides, protamex was selected for the hydrolysis of sea cucumber viscera. One-factor-at-a-time method was used to investigate the effects of temperature, pH, enzyme dosage, and hydrolysis time on hydrolysis degree. Following these investigation, a quadratic regression model describing hydrolysis degree as a response to s tem-perature, pH, enzyme dosage, and hydrolysis time was set up using a Box-Behnken experimental design. As dem-onstrated by the results analysis of variance and validation experiments, the model could well indicate the hydroly-sis degree of sea cucumber viscera. The optimal hydrolysis conditions of temperature, pH, enzyme dosage, and hy-drolysis time were determined to be 52.66℃, 6.71, 2.5% (dry mass ratio) and 10 hour, respectively. Under these conditions, the hydrolysis degree of sea cucumber viscera is 49.58%. Under the best condition of enzymatic hy-drolysis, more than 74%of the peptides were less than 500 Da. The antioxidant activity of peptides was measured by DPPH method, showing that the radical scavenging activity is 24.8%when the concentration was about 3 mg/mL. The antioxidant activity of peptides was measured by ABTS method, showing that the Vitamin C Equivalent Anti-oxidant Capacity is 0.255μmol/g when the concentration was about 3 mg/mL.