CAJ | 학술논문

为检测中国近海海洋环境中菊酯类农药等污染物对日本囊对虾(Marsupenaeus japonicas)的基因毒性及其危害程度，作者首次使用醚菊酯对日本囊对虾进行毒理实验，将日本囊对虾分为6组进行不同剂量的处理，采用彗星实验分析技术对处理培育25 d的日本囊对虾DNA损伤情况进行检测，并通过Comet Score 1.5分析软件对实验图谱进行拖尾率、尾长、彗尾DNA相对含量、尾距、Olive距等DNA损伤指标的统计分析，在对各指标分别建立一元回归方程的基础上，又通过 SPSS 软件进行判定分析，筛选影响作用更大的检测指标，建立了多参数的多元回归方程。实验结果发现，与空白对照组相比，日本囊对虾各实验处理组肝胰腺细胞 DNA 均有不同程度的损伤且各检测指标均有显著性差异(P<0.01)，各检测指标与处理浓度表现出良好的剂量效应关系，随着处理浓度递增，各检测指标都呈规律性的增长趋势。回归分析和相关系数表明：各检测指标与处理浓度具有高度的相关性，多项式回归方程(P<0.05)和多元回归方程(P<0.005)均有显著性的统计学意义，通过多元回归方程可以有效地推断醚菊酯处理浓度和处理时间。
위검측중국근해해양배경중국지류농약등오염물대일본낭대하(Marsupenaeus japonicas)적기인독성급기위해정도，작자수차사용미국지대일본낭대하진행독리실험，장일본낭대하분위6조진행불동제량적처리，채용혜성실험분석기술대처리배육25 d적일본낭대하DNA손상정황진행검측，병통과Comet Score 1.5분석연건대실험도보진행타미솔、미장、혜미DNA상대함량、미거、Olive거등DNA손상지표적통계분석，재대각지표분별건립일원회귀방정적기출상，우통과 SPSS 연건진행판정분석，사선영향작용경대적검측지표，건립료다삼수적다원회귀방정。실험결과발현，여공백대조조상비，일본낭대하각실험처리조간이선세포 DNA 균유불동정도적손상차각검측지표균유현저성차이(P<0.01)，각검측지표여처리농도표현출량호적제량효응관계，수착처리농도체증，각검측지표도정규률성적증장추세。회귀분석화상관계수표명：각검측지표여처리농도구유고도적상관성，다항식회귀방정(P<0.05)화다원회귀방정(P<0.005)균유현저성적통계학의의，통과다원회귀방정가이유효지추단미국지처리농도화처리시간。
In order to analyze the genotoxicity of Marsupenaeus japonicas caused by the pollutant of pyrethroid pesticides in the coastal sea of China, here, we applied ethofenprox in toxicological experiment on M. japonicas for the first time. In our study, M. japonicas were divided into six groups and exposed to different concentrations of ethofenprox for 25 days. DNA damage in hepatopancreas cells of M. japonicas was evaluated with comet assay. The DNA damage parameters such as the comet rate, the tail length, the percentage of tail DNA, the tail moment and the olive moment were statistically analyzed by Comet Score 1.5. On the basis of one-dimensional regression equation, we selected greater influenced parameters after comparing with SPSS 16.0 and constructed the multiple- dimen-sional regression equation. The results show that DNA damage in hepatopancreas cells was observed in all exposed groups and the parameters of each exposed group were significantly different from the control group (P<0.01). There was a dose-effect relationship between ethofenprox and DNA damage in hepatopancreas cells, which in-creased regularly with the exposure concentrations. The regression analysis and coefficient indicated that all DNA damage parameters were highly relevant to the exposed concentrations. The one-dimensional regression equation (P<0.05) and multiple-dimensional regression equation (P<0.005) had significant statistic meaning, which might be an effective way to estimate the exposed concentration and time of ethofenprox.