CAJ | 학술논문

目的：：建立恩施产杜仲药材水提液HPLC指纹图谱的分析方法。方法：10批恩施产杜仲药材水提,采用WondaSil C18(250 mm ×4.6 mm,5μm)色谱柱,以乙腈-0.1%磷酸水溶液二元线性梯度洗脱,检测波长230 nm,流速1.0 ml·min-1,柱温25℃,进样量10μl,进行 HPLC指纹图谱分析。结果：确定了12个共有峰,采用《中药色谱指纹图谱相似度评价系统(2004A版)》计算10批杜仲药材水提液的指纹图谱整体相似度在0.596~0.997,并得出对照指纹图谱。结论：针对恩施产杜仲药材建立的HPLC指纹图谱分析方法,操作简便,稳定性高,重复性好,可有效地控制恩施产杜仲药材的内在质量。
목적：：건립은시산두중약재수제액HPLC지문도보적분석방법。방법：10비은시산두중약재수제,채용WondaSil C18(250 mm ×4.6 mm,5μm)색보주,이을정-0.1%린산수용액이원선성제도세탈,검측파장230 nm,류속1.0 ml·min-1,주온25℃,진양량10μl,진행 HPLC지문도보분석。결과：학정료12개공유봉,채용《중약색보지문도보상사도평개계통(2004A판)》계산10비두중약재수제액적지문도보정체상사도재0.596~0.997,병득출대조지문도보。결론：침대은시산두중약재건립적HPLC지문도보분석방법,조작간편,은정성고,중복성호,가유효지공제은시산두중약재적내재질량。
Objective:To establish the fingerprint analysis method for the aqueous extracts of Eucommia ulmoides from Enshi by HPLC. Methods:The fingerprint of aqueous extracts of ten batches of Eucommia ulmoides from Enshi were analyzed by HPLC. The columnwasWondaSilC18(250mm×4.6mm,5μm). Themobilephaseconsistedofacetonitrile-0.1% phosphoricacidwithgradient elution. The flow rate was 1. 0 ml·min-1 , the detection wavelength was 230 nm, the column temperature was 25℃, and the injection volume was 10 μl. Results:The fingerprint consisted of 12 common peaks. The similarity range of ten batches of Eucommia ulmoides calculated by similarity evaluation system for the chromatographic fingerprint of TCM(Version 2004 A)was 0. 596-0. 997. The standard fingerprint of Eucommia ulmoides was established by HPLC. Conclusion: The established HPLC fingerprint analysis method for Eu-commia ulmoides from Enshi is simple, stable and reproducible, which can effectively control the quality of Eucommia ulmoides from Enshi.