中国药师
中國藥師
중국약사
CHINA PHARMACIST
2014年
12期
2067-2070,2074
,共5页
黄良永%叶正德%郑江萍%杨光义
黃良永%葉正德%鄭江萍%楊光義
황량영%협정덕%정강평%양광의
连翘%指纹图谱%高效液相色谱法%连翘酯苷A%连翘苷
連翹%指紋圖譜%高效液相色譜法%連翹酯苷A%連翹苷
련교%지문도보%고효액상색보법%련교지감A%련교감
Forsytiae suspensa Fructus%Fingerprint%HPLC%Forsythoside A%Forsythin
目的：：建立十堰地区连翘药材化学成分的HPLC指纹特征图谱,为连翘药材的质量控制提供科学依据。方法：采用HPLC-梯度洗脱法,以Waters SunFire C18(250 mm ×4.6 mm,5μm)为色谱柱,Diamonsil C18保护柱,以乙腈和0.4%醋酸溶液为流动相,梯度洗脱,流速为1 ml·min-1,检测波长为277 nm,柱温为30℃。结果：不同产地的12批连翘药材化学成分特征图谱共检出14个共有峰。对不同产地的14个特征峰进行聚类分析,连翘药材产地与特征图谱相似度差异不大,样品中连翘酯苷A和连翘苷的含量有明显差别。结论：该方法简便、快速,可做为连翘药材的质量控制的有效手段。
目的：：建立十堰地區連翹藥材化學成分的HPLC指紋特徵圖譜,為連翹藥材的質量控製提供科學依據。方法：採用HPLC-梯度洗脫法,以Waters SunFire C18(250 mm ×4.6 mm,5μm)為色譜柱,Diamonsil C18保護柱,以乙腈和0.4%醋痠溶液為流動相,梯度洗脫,流速為1 ml·min-1,檢測波長為277 nm,柱溫為30℃。結果：不同產地的12批連翹藥材化學成分特徵圖譜共檢齣14箇共有峰。對不同產地的14箇特徵峰進行聚類分析,連翹藥材產地與特徵圖譜相似度差異不大,樣品中連翹酯苷A和連翹苷的含量有明顯差彆。結論：該方法簡便、快速,可做為連翹藥材的質量控製的有效手段。
목적：：건립십언지구련교약재화학성분적HPLC지문특정도보,위련교약재적질량공제제공과학의거。방법：채용HPLC-제도세탈법,이Waters SunFire C18(250 mm ×4.6 mm,5μm)위색보주,Diamonsil C18보호주,이을정화0.4%작산용액위류동상,제도세탈,류속위1 ml·min-1,검측파장위277 nm,주온위30℃。결과：불동산지적12비련교약재화학성분특정도보공검출14개공유봉。대불동산지적14개특정봉진행취류분석,련교약재산지여특정도보상사도차이불대,양품중련교지감A화련교감적함량유명현차별。결론：해방법간편、쾌속,가주위련교약재적질량공제적유효수단。
Objective:To establish the HPLC fingerprints of chemical constituents in Forsytiae suspensa Fructus from Shiyan dis-trict to provide scientific evidence for the quality control of Forsytiae suspensa Fructus. Methods: HPLC was performed on a Waters SunFire C18 (250 mm × 4. 6 mm, 5 μm) column and a Diamonsil C18 guard column, and the mobile phase consisted of acetonitrile-0. 4% acetic acid solution with gradient elution. The flow rate was 1 ml·min-1 , the detection wavelength was 277 nm, and the col-umn temperature was 30℃. Results:There were 14 common peaks in the HPLC fingerprints of chemical constituents in 12 batches of Forsytiae suspensa Fructus from Shiyan. The 14 characteristic peaks of Forsytiae suspensa Fructus from different habitats were under clustering analysis, and the similarity showed little difference. The contents of forsythoside A and forsythin exhibited significant differ-ence in the samples. Conclusion:The HPLC fingerprint method is simple, rapid and feasible in the quality control of Forsytiae suspen-sa Fructus.
