中国药师
中國藥師
중국약사
CHINA PHARMACIST
2014年
12期
2063-2066
,共4页
杨光义%刘泽干%叶方%杜婷%杜士明
楊光義%劉澤榦%葉方%杜婷%杜士明
양광의%류택간%협방%두정%두사명
苍术%指纹图谱%质量控制%高效液相色谱法
蒼術%指紋圖譜%質量控製%高效液相色譜法
창술%지문도보%질량공제%고효액상색보법
Atractylodes lancea%Fingerprints%Quality control%HPLC
目的：：建立鄂西北地区苍术药材HPLC指纹图谱,为苍术药材质量控制及品种鉴定提供依据。方法：采用HPLC法,以Diamonsil2# C18(250 mm ×4.6 mm,5μm)色谱柱,Diamonsil预柱,流动相为乙腈-水,梯度洗脱,流速：1.0 ml·min-1,检测波长：340 nm。结果：14批苍术药材特征图谱共检出13个分离度良好的共有峰；将13个共有峰进行聚类分析,鄂西北地区苍术聚为两大类。结论：该方法简便、快捷,可作为苍术药材质量控制的有利手段。
目的：：建立鄂西北地區蒼術藥材HPLC指紋圖譜,為蒼術藥材質量控製及品種鑒定提供依據。方法：採用HPLC法,以Diamonsil2# C18(250 mm ×4.6 mm,5μm)色譜柱,Diamonsil預柱,流動相為乙腈-水,梯度洗脫,流速：1.0 ml·min-1,檢測波長：340 nm。結果：14批蒼術藥材特徵圖譜共檢齣13箇分離度良好的共有峰；將13箇共有峰進行聚類分析,鄂西北地區蒼術聚為兩大類。結論：該方法簡便、快捷,可作為蒼術藥材質量控製的有利手段。
목적：：건립악서북지구창술약재HPLC지문도보,위창술약재질량공제급품충감정제공의거。방법：채용HPLC법,이Diamonsil2# C18(250 mm ×4.6 mm,5μm)색보주,Diamonsil예주,류동상위을정-수,제도세탈,류속：1.0 ml·min-1,검측파장：340 nm。결과：14비창술약재특정도보공검출13개분리도량호적공유봉；장13개공유봉진행취류분석,악서북지구창술취위량대류。결론：해방법간편、쾌첩,가작위창술약재질량공제적유리수단。
Objective:To establish the HPLC fingerprints of Atractylodes lancea in northwest of Hubei province to provide the basis for the quality control and variety identification of Atractylodes lancea. Methods:The fingerprints were detected by HPLC. The separa-tion was achieved on a Diamonsil 2# C18(250 mm ×4.6 mm,5 μm)chromatographic column at 30℃ using acetonitrile-water (gradient elution) as the mobile phase at a flow rate of 1. 0 ml·min-1 . The detection wavelength was 340nm. Results: There were 13 common peaks in the HPLC fingerprints of 14 batches of Atractylodes lancea with promising separation. According to the results of clustering a-nalysis,Atractylodes lancea in northwest of Hubei province was clustered into two categories. Conclusion:A simple, feasible and relia-ble method is provided for the quality control of Atractylodes lancea.
