中国药师
中國藥師
중국약사
CHINA PHARMACIST
2014年
12期
2016-2018
,共3页
美罗培南%曙红Y%荧光素%尿液%荧光共振能量转移光谱法
美囉培南%曙紅Y%熒光素%尿液%熒光共振能量轉移光譜法
미라배남%서홍Y%형광소%뇨액%형광공진능량전이광보법
Meropenem%Eosin-Y%Fluorescein%Urine%Fluorescence resonance energy transfer spectroscopy
目的：：建立荧光共振能量转移光谱法测定尿液中美罗培南的含量。方法：采用LS-55型荧光分光光度计,于10 ml比色管中,依次加入美罗培南溶液、荧光素溶液、曙红Y溶液、BR缓冲溶液和十六烷基三甲基溴化铵,于λex=455 nm,λem=547 nm处,测定体系和样品试剂空白的荧光强度F和F0,以ΔF=F-F0作为测定美罗培南的信号响应值,计算其含量。结果：尿液中美罗培南的线性回归方程：ΔF=33.8C+53.4, r=0.9917,线性范围为0.5~10μg·ml-1,检出限为0.13μg· ml-1,加标回收率为98.9%~103.0%,RSD为0.3%~0.4%。结论：该方法灵敏准确,适用于美罗培南临床药物动力学的研究。
目的：：建立熒光共振能量轉移光譜法測定尿液中美囉培南的含量。方法：採用LS-55型熒光分光光度計,于10 ml比色管中,依次加入美囉培南溶液、熒光素溶液、曙紅Y溶液、BR緩遲溶液和十六烷基三甲基溴化銨,于λex=455 nm,λem=547 nm處,測定體繫和樣品試劑空白的熒光彊度F和F0,以ΔF=F-F0作為測定美囉培南的信號響應值,計算其含量。結果：尿液中美囉培南的線性迴歸方程：ΔF=33.8C+53.4, r=0.9917,線性範圍為0.5~10μg·ml-1,檢齣限為0.13μg· ml-1,加標迴收率為98.9%~103.0%,RSD為0.3%~0.4%。結論：該方法靈敏準確,適用于美囉培南臨床藥物動力學的研究。
목적：：건립형광공진능량전이광보법측정뇨액중미라배남적함량。방법：채용LS-55형형광분광광도계,우10 ml비색관중,의차가입미라배남용액、형광소용액、서홍Y용액、BR완충용액화십륙완기삼갑기추화안,우λex=455 nm,λem=547 nm처,측정체계화양품시제공백적형광강도F화F0,이ΔF=F-F0작위측정미라배남적신호향응치,계산기함량。결과：뇨액중미라배남적선성회귀방정：ΔF=33.8C+53.4, r=0.9917,선성범위위0.5~10μg·ml-1,검출한위0.13μg· ml-1,가표회수솔위98.9%~103.0%,RSD위0.3%~0.4%。결론：해방법령민준학,괄용우미라배남림상약물동역학적연구。
Objective: To establish the determination method for meropenem in urine by fluorescence resonance energy transfer spectroscopy. Methods:An LS-55 fluorescence spectrophotometer was used. The solution of meropenem, fluorescein, eosin Y, BR buffer and cetyl trimethyl ammonium bromide was respectively added into a 10 ml colorimetric tube. At λex of 455 nm and λem of 547 nm, the fluorescence intensity F and F0 of the system and the reagent blank sample was detected. Using ΔF = F-F0 as signal re-sponse value for meropenem, the content was calculated. Results: The linear regression equation of meropenem in urine was as fol-lows:ΔF=33. 8C+53. 4 (r=0. 991 7), the linear range was 0. 5-10 μg·ml-1 with the detection limit of 0. 13 μg·ml-1, the re-covery of 98. 9%-103. 0% and RSD of 0. 3%-0. 4%. Conclusion:The method is rapid and accurate, which can be used in the phar-macokinetic study of meropenem.
