CAJ | 학술논문

目的：：比较酶放大免疫( EMIT)法和HPLC法测定人全血环孢素( CsA)浓度的区别与相关性。方法：收集肾移植患者服药后的稳态谷浓度血样119份,分别用 EMIT法和 HPLC法进行测定,考察两种测定方法的区别和相关程度。结果：HPLC法和EMIT法检测的CsA血浓度差异有统计学意义(P<0.05),EMIT法测定全血CsA浓度的结果较HPLC法测定的结果平均高26.2 ng·ml-1,95%CI为(14.6~37.7)ng·ml-1,两种分析方法具有良好的相关性(r=0.9974)。结论：HPLC法和EMIT法测定CsA全血药物浓度结果差异具有统计学意义,在临床进行CsA药物浓度监测中应予以关注并作相应调整。
목적：：비교매방대면역( EMIT)법화HPLC법측정인전혈배포소( CsA)농도적구별여상관성。방법：수집신이식환자복약후적은태곡농도혈양119빈,분별용 EMIT법화 HPLC법진행측정,고찰량충측정방법적구별화상관정도。결과：HPLC법화EMIT법검측적CsA혈농도차이유통계학의의(P<0.05),EMIT법측정전혈CsA농도적결과교HPLC법측정적결과평균고26.2 ng·ml-1,95%CI위(14.6~37.7)ng·ml-1,량충분석방법구유량호적상관성(r=0.9974)。결론：HPLC법화EMIT법측정CsA전혈약물농도결과차이구유통계학의의,재림상진행CsA약물농도감측중응여이관주병작상응조정。
Objective:To compare the difference and correlation of HPLC and enzyme-multiplied immunoassay test( EMIT) for the determination of CsA in human whole blood. Methods:A total of 119 clinical samples at different concentrations of CsA were collected and respectively determined by HPLC and EMIT. The difference and correlation of the two determination methods were investigated. Results:There was significant difference in the blood concentrations of CsA determined by HPLC and EMIT(P<0. 05). CsA concen-tration determined by EMIT was 26. 2 ng·ml-1 higher than that determined by HPLC, and 95% CI was (14. 6-37. 7) ng·ml-1 . A satisfactory correlation was achieved between the two methods(r=0. 997 4). Conclusion:There is statistically significant difference in the CsA concentration in whole blood respectively determined by EMIT and HPLC. Attention should be paid to CsA monitoring by E-MIT and HPLC, and relevant adjustment should be carried out.