中国药师
中國藥師
중국약사
CHINA PHARMACIST
2014年
12期
2151-2153
,共3页
矮地茶%岩白菜素%毛细管电泳%含量测定
矮地茶%巖白菜素%毛細管電泳%含量測定
왜지다%암백채소%모세관전영%함량측정
Herba Ardisiae Japonicae%Bergeni%Capilary zone electrophoresis%Determination
目的：：建立矮地茶药材中岩白菜素的含量测定方法。方法：矮地茶药材粉碎后过筛,取粉末用甲醇超声提取,未涂渍熔融石英毛细管(75μm ×48.5 cm,有效长度40 cm),以25 mmol·L-1硼砂水溶液为缓冲液,运行电压15 kV,温度25℃,内标为肉桂酸,检测波长为240 nm,运行时间10 min。结果：在此条件下岩白菜素与内标及其他成分能达到基线分离,岩白菜素在3.356~167.800μg·ml-1范围内呈良好的线性关系 Y =0.0349X +0.0606,r =0.9979,最低检测限为0.839μg· ml-1(S/N =3),最低定量限为3.356μg·ml-1(S/N =10),平均加样回收率为99.6%(RSD =3.0%,n =6),低,中,高浓度日内、日间精密度RSD均小于5%(n =3)。结论：此方法可用于矮地茶药材中岩白菜素的含量测定,具有简便快速、准确可靠、试剂消耗量少的优点。
目的：：建立矮地茶藥材中巖白菜素的含量測定方法。方法：矮地茶藥材粉碎後過篩,取粉末用甲醇超聲提取,未塗漬鎔融石英毛細管(75μm ×48.5 cm,有效長度40 cm),以25 mmol·L-1硼砂水溶液為緩遲液,運行電壓15 kV,溫度25℃,內標為肉桂痠,檢測波長為240 nm,運行時間10 min。結果：在此條件下巖白菜素與內標及其他成分能達到基線分離,巖白菜素在3.356~167.800μg·ml-1範圍內呈良好的線性關繫 Y =0.0349X +0.0606,r =0.9979,最低檢測限為0.839μg· ml-1(S/N =3),最低定量限為3.356μg·ml-1(S/N =10),平均加樣迴收率為99.6%(RSD =3.0%,n =6),低,中,高濃度日內、日間精密度RSD均小于5%(n =3)。結論：此方法可用于矮地茶藥材中巖白菜素的含量測定,具有簡便快速、準確可靠、試劑消耗量少的優點。
목적：：건립왜지다약재중암백채소적함량측정방법。방법：왜지다약재분쇄후과사,취분말용갑순초성제취,미도지용융석영모세관(75μm ×48.5 cm,유효장도40 cm),이25 mmol·L-1붕사수용액위완충액,운행전압15 kV,온도25℃,내표위육계산,검측파장위240 nm,운행시간10 min。결과：재차조건하암백채소여내표급기타성분능체도기선분리,암백채소재3.356~167.800μg·ml-1범위내정량호적선성관계 Y =0.0349X +0.0606,r =0.9979,최저검측한위0.839μg· ml-1(S/N =3),최저정량한위3.356μg·ml-1(S/N =10),평균가양회수솔위99.6%(RSD =3.0%,n =6),저,중,고농도일내、일간정밀도RSD균소우5%(n =3)。결론：차방법가용우왜지다약재중암백채소적함량측정,구유간편쾌속、준학가고、시제소모량소적우점。
Objective:To establish a method for the determination of bergeni in Herba Ardisiae Japonicae. Methods: Herba Ardisiae Japonicae was ultrasonic extracted by methanol after crushed and sieved. An untreated fused-silica capillary (75 μm × 48. 5 cm, 40 cm effective length) was used, the running buffer was 25 mmol·L-1 borax in water, the voltage was 15 kV, the temperature was 25℃, the internal standard was cinnamic acid, the ultraviolet wavelength was set at 240nm, and the running time was 10 minute. Results:Bergeni, the internal standard and the impurities were separated by baseline under the above conditions. Bergeni was linear within the range of 3. 356-167. 800μg·ml-1 with the linear equation of Y =0. 034 9X+0. 060 6(r =0. 997 9), the limit of determination was 0. 839 μg·ml-1(S/N = 3), the limit of quantitation was 3. 356 μg·ml-1(S/N = 10), the average recovery was 99. 6%(RSD = 3. 1%, n = 6), and RSDs of intra and inter-day were lower than 5% (n = 3). Conclusion: The method has the advantages of convenience, fastness, accuracy, and low sample and reagent consumption, which can be used to determine bergeni in Herba Ardisiae Japonicae.
