CAJ | 학술논문

目的：通过观察不同时程电针预处理对脑缺血大鼠脑含水量及血脑屏障通透性的影响,评价电针预处理对脑缺血大鼠血脑屏障功能的保护效应。方法将清洁级健康雄性SD大鼠随机分为5组(n=48),模型对照组、电针预处理1 d组、电针预处理3 d组、电针预处理7 d组及电针预处理15 d组,电针预处理各组电针预处理结束后建立大鼠单侧大脑中动脉阻塞(MCAO)模型,分别于脑缺血12 h、24 h和48 h,检测脑含水量、血脑屏障EB透过量。结果模型对照组脑缺血12 h和48 h时间点大鼠脑含水量及血脑屏障EB透过量均低于脑缺血24 h时间点(P＜0.05)；电针预处理15 d组在脑缺血各时间点对脑含水量及EB透过量的减少作用显著(P＜0.05,P＜0.01,P＜0.001)。结论脑缺血12 h、24 h、48 h3个时间点中以脑缺血24 h时间点血脑屏障损伤程度显著；电针预处理可通过明显降低脑缺血大鼠脑水肿及血脑屏障通透性发挥其对脑缺血大鼠血脑屏障功能的保护效应。
목적：통과관찰불동시정전침예처리대뇌결혈대서뇌함수량급혈뇌병장통투성적영향,평개전침예처리대뇌결혈대서혈뇌병장공능적보호효응。방법장청길급건강웅성SD대서수궤분위5조(n=48),모형대조조、전침예처리1 d조、전침예처리3 d조、전침예처리7 d조급전침예처리15 d조,전침예처리각조전침예처리결속후건립대서단측대뇌중동맥조새(MCAO)모형,분별우뇌결혈12 h、24 h화48 h,검측뇌함수량、혈뇌병장EB투과량。결과모형대조조뇌결혈12 h화48 h시간점대서뇌함수량급혈뇌병장EB투과량균저우뇌결혈24 h시간점(P＜0.05)；전침예처리15 d조재뇌결혈각시간점대뇌함수량급EB투과량적감소작용현저(P＜0.05,P＜0.01,P＜0.001)。결론뇌결혈12 h、24 h、48 h3개시간점중이뇌결혈24 h시간점혈뇌병장손상정도현저；전침예처리가통과명현강저뇌결혈대서뇌수종급혈뇌병장통투성발휘기대뇌결혈대서혈뇌병장공능적보호효응。
ObjectiveTo investigate the impactsof different time-course electroacupuncture pretreatments on brain water content and blood-brain barrier permeability and assess their protecting effects on blood-brain barrier functionin cerebral ischemia rats. MethodClean-grade healthy male SD rats were randomly allocated to five groups (n=48): model control group, and one-day, three-day, seven-day and fifteen-day electroacupuncture pretreatment groups. A rat model of unilateral middle cerebral artery occlusion (MCAO) was made after electroacupuncture pretreatment was finished in various electroacupuncture groups. The water content of the brain and the amount of EB through blood brain barrier were measured at 12, 24 and 48 hrs after cerebral ischemia. ResultBoth brain water content and EB permeation amount were lower at 12 and 48 hrs than at 24 hrs after cerebral ischemia (P<0.05). There was a significant reducing effect on brain water content and EB permeation amount in the fifteen-day electroacupuncture pretreatment group at various time points after cerebral ischemia (P<0.05,P<0.01,P<0.001).Conclusion The blood-brain barrier is impaired most severely at 24 hrs of the three time points 12, 24 and 48 hrs after cerebral ischemia. Electroacupuncture pretreatment can produce a protecting effect on blood-brain barrier function in cerebral ischemia rats by markedly reducing cerebral edema and blood-brain barrier permeability.