药学研究
藥學研究
약학연구
JOURNAL OF PHARMACEUTICAL RESEARCH
2014年
12期
689-690,695
,共3页
张新军%梁瑞雪%侯文静%贾元印
張新軍%樑瑞雪%侯文靜%賈元印
장신군%량서설%후문정%가원인
痛经停膏%丹酚酸B%含量测定%高效液相色谱法
痛經停膏%丹酚痠B%含量測定%高效液相色譜法
통경정고%단분산B%함량측정%고효액상색보법
Tongjingting Ointment%Salvianolic acid B%Determination%HPLC
目的:建立痛经停膏中丹酚酸B的含量测定方法。方法采用高效液相色谱法,色谱柱为Diamonsil C18(4.6 mm ×250 mm,5μm),流动相为甲醇-乙腈-甲酸-水(30:10:1:59),流速为1.0 mL·min-1,测定波长为286 nm。结果丹酚酸B在0.525~2.625μg范围内与色谱峰峰面积呈良好线性关系,相关系数为0.9999,平均加样回收率为98.2%,RSD为0.81%。结论本方法简便可靠,准确灵敏,专属性强,能有效的控制痛经停膏的内在质量。
目的:建立痛經停膏中丹酚痠B的含量測定方法。方法採用高效液相色譜法,色譜柱為Diamonsil C18(4.6 mm ×250 mm,5μm),流動相為甲醇-乙腈-甲痠-水(30:10:1:59),流速為1.0 mL·min-1,測定波長為286 nm。結果丹酚痠B在0.525~2.625μg範圍內與色譜峰峰麵積呈良好線性關繫,相關繫數為0.9999,平均加樣迴收率為98.2%,RSD為0.81%。結論本方法簡便可靠,準確靈敏,專屬性彊,能有效的控製痛經停膏的內在質量。
목적:건립통경정고중단분산B적함량측정방법。방법채용고효액상색보법,색보주위Diamonsil C18(4.6 mm ×250 mm,5μm),류동상위갑순-을정-갑산-수(30:10:1:59),류속위1.0 mL·min-1,측정파장위286 nm。결과단분산B재0.525~2.625μg범위내여색보봉봉면적정량호선성관계,상관계수위0.9999,평균가양회수솔위98.2%,RSD위0.81%。결론본방법간편가고,준학령민,전속성강,능유효적공제통경정고적내재질량。
Objective To establish a method for the determination of salvianolic acid B in Tongjingting Oint-ment. Methods The HPLC procedure was performed on the chromatographic column of Diamonsil C18(4. 6 mm × 250 mm, 5 μm),the mobile phase was a mixture of methanol-acetonitrile-formic acid-water(30:10:1:59),the flow rate was 1. 0 mL·min-1 and the detection wavelength was 286 nm. Results Salvianolic acid B showed a good relationship with peak ar-ea at a range from 0. 525 μg to 2. 625 μg,and the correlation coefficients were 0. 999 9,the average recovery of scutellarin was 98. 2%( RSD=0. 81%). Conclusion The method was simple and reliable,accurate and sensitive and specific which can be used effectively for the quality control of Tongjingting Ointment.