CAJ | 학술논문

目的：研究半边旗有效成分贝壳杉烷型二萜类化合物5F对腹膜炎小鼠血清一氧化氮（NO）和细胞因子IL-6、IL-10、TNF-α、MCP-1的影响。方法预先给予50和100 mg·kg-15F溶液3 d后，腹腔注射50 mg·kg-1酵母多糖建立腹膜炎模型，Griess法测一氧化氮含量，流式细胞术检测IL-6、IL-10、TNF-α和MCP-1的水平。结果与模型组比较，5F可以降低腹膜炎小鼠血清一氧化氮水平（ P﹤0.05），减少IL-6、IL-10、TNF-α和MCP-1含量（P﹤0.05或P﹤0.01）。结论5F可以有效减少酵母多糖诱导的腹膜炎小鼠血清一氧化氮、TNF-α、IL-6、IL-10和MCP-1含量。
목적：연구반변기유효성분패각삼완형이첩류화합물5F대복막염소서혈청일양화담（NO）화세포인자IL-6、IL-10、TNF-α、MCP-1적영향。방법예선급여50화100 mg·kg-15F용액3 d후，복강주사50 mg·kg-1효모다당건립복막염모형，Griess법측일양화담함량，류식세포술검측IL-6、IL-10、TNF-α화MCP-1적수평。결과여모형조비교，5F가이강저복막염소서혈청일양화담수평（ P﹤0.05），감소IL-6、IL-10、TNF-α화MCP-1함량（P﹤0.05혹P﹤0.01）。결론5F가이유효감소효모다당유도적복막염소서혈청일양화담、TNF-α、IL-6、IL-10화MCP-1함량。
Objective To investigate the effect of 5F,an extract from Pteris semipinnata L. serum NO and cytokine levels in zymosan A-induced peritonitis mice. Methods After pretreatment with 50 and 100 mg·kg-1 5F solution for 3 d,mice were intraperitoneal injection of 50 mg·kg-1 zymosan to induce peritonitis. The release of nitric oxide was meas-ured by a Griess reagent system. The serum levels of IL-6,IL-10,TNF-αand MCP-1 were determined by flow cytome-try. Results Compared with model group,5F pretreatment could decrease the overproduction of NO,IL-6,IL-10,TNF-αand MCP-1(P﹤0. 05 or P﹤0. 01). Conclusion 5F could significantly reduce the serum level of NO,TNF- α,IL-6,IL-10 and MCP-1 in zymosan A-induced peritonitis mice.