CAJ | 학술논문

目的：获得徐州地区猫源弓形虫虫株，了解徐州地区流浪猫弓形虫感染情况。方法捕捉徐州地区流浪猫，收集血清后，用ELISA试剂盒检测弓形虫特异性IgG抗体；同时将猫的心、脑、舌用盐酸?胃蛋白酶溶液消化处理后接种小鼠，经连续传代分离弓形虫虫株，采用特异PCR法对所分离的虫株进行鉴定。结果共捕捉41只流浪猫。从猫体内共分离到弓形虫虫株11株，PCR均扩增出弓形虫特异性条带；ELISA结果表明41.5%的流浪猫均能检测到弓形虫抗体。结论徐州地区流浪猫弓形虫感染较为严重，有可能成为人和其它动物弓形虫病的重要传染源，应引起相关部门的重视。
목적：획득서주지구묘원궁형충충주，료해서주지구류랑묘궁형충감염정황。방법포착서주지구류랑묘，수집혈청후，용ELISA시제합검측궁형충특이성IgG항체；동시장묘적심、뇌、설용염산?위단백매용액소화처리후접충소서，경련속전대분리궁형충충주，채용특이PCR법대소분리적충주진행감정。결과공포착41지류랑묘。종묘체내공분리도궁형충충주11주，PCR균확증출궁형충특이성조대；ELISA결과표명41.5%적류랑묘균능검측도궁형충항체。결론서주지구류랑묘궁형충감염교위엄중，유가능성위인화기타동물궁형충병적중요전염원，응인기상관부문적중시。
Objective To isolate Toxoplasma gondii T. gondii strains from stray cats and explore their prevalence in Xu?zhou City. Methods The sera of 41 stray cats were collected to detect the antibodies of T. gondii by using a commercial enzyme?linked immunosorbent ELISA kit. The tissues including the heart brain and tongue from these cats were digested by acid pep?sin solution and inoculated to Kunming mice. These suspicious isolates were subsequently identified by a specific PCR method. Results A total of 11 strains were isolated from 41 stray cats which were confirmed by the PCR results. Moreover 17 cats 41.5% were found to be positive with the antibodies of T. gondii. Conclusion A high prevalence of T. gondii infection was found in Xuzhou City which indicates that the stray cats infected with T. gondii would be an important infection source that may infect humans and other animals in this area.