中国血吸虫病防治杂志
中國血吸蟲病防治雜誌
중국혈흡충병방치잡지
CHINESE JOURNAL OF SCHISTOSOMIASIS CONTROL
2014年
6期
632-635
,共4页
日本血吸虫%泥蚶%多糖%佐剂%DNA疫苗
日本血吸蟲%泥蚶%多糖%佐劑%DNA疫苗
일본혈흡충%니감%다당%좌제%DNA역묘
Schistosoma japonicum%Tegillarca granosa%Polysaccharides%Adjuvant%DNA vaccine
目的：探讨泥蚶多糖佐剂对日本血吸虫DNA疫苗免疫效果的影响。方法将60只雌性BALB/c小鼠随机分为对照组、泥蚶多糖组、疫苗组、疫苗+佐剂组等4组，每组15只，分别皮下注射100μl PBS、100μg泥蚶多糖、100μg pcD?NA3.1?Sj26GST血吸虫DNA疫苗、100μg pcDNA3.1?Sj26GST与等量泥蚶多糖佐剂的混合物。各组分别于实验开始时、第2周、第4周各免疫1次。末次免疫后第2周，以日本血吸虫尾蚴40±1条/只经腹部皮肤攻击感染小鼠。感染后6周剖杀小鼠，收集血清、肝脏及成虫，以ELISA法测定血清中特异性IgG水平，计算减虫率及每克肝组织减卵率。结果感染后6周，疫苗组和疫苗+佐剂组小鼠血清中IgG抗体水平分别为18.26±0.42 mg/ml和20.21±0.89 mg/ml，差异有统计学意义（P<0.05），两组均显著高于对照组（P均<0.05）；疫苗组减虫率和减卵率分别为28.60%和35.84%，疫苗+佐剂组减虫率和减卵率分别为38.04%和49.74%，差异均有统计学意义（P均<0.05），且均显著高于PBS对照组（P均<0.01）。结论泥蚶多糖作为佐剂，对日本血吸虫DNA疫苗具有明显增效作用。
目的：探討泥蚶多糖佐劑對日本血吸蟲DNA疫苗免疫效果的影響。方法將60隻雌性BALB/c小鼠隨機分為對照組、泥蚶多糖組、疫苗組、疫苗+佐劑組等4組，每組15隻，分彆皮下註射100μl PBS、100μg泥蚶多糖、100μg pcD?NA3.1?Sj26GST血吸蟲DNA疫苗、100μg pcDNA3.1?Sj26GST與等量泥蚶多糖佐劑的混閤物。各組分彆于實驗開始時、第2週、第4週各免疫1次。末次免疫後第2週，以日本血吸蟲尾蚴40±1條/隻經腹部皮膚攻擊感染小鼠。感染後6週剖殺小鼠，收集血清、肝髒及成蟲，以ELISA法測定血清中特異性IgG水平，計算減蟲率及每剋肝組織減卵率。結果感染後6週，疫苗組和疫苗+佐劑組小鼠血清中IgG抗體水平分彆為18.26±0.42 mg/ml和20.21±0.89 mg/ml，差異有統計學意義（P<0.05），兩組均顯著高于對照組（P均<0.05）；疫苗組減蟲率和減卵率分彆為28.60%和35.84%，疫苗+佐劑組減蟲率和減卵率分彆為38.04%和49.74%，差異均有統計學意義（P均<0.05），且均顯著高于PBS對照組（P均<0.01）。結論泥蚶多糖作為佐劑，對日本血吸蟲DNA疫苗具有明顯增效作用。
목적：탐토니감다당좌제대일본혈흡충DNA역묘면역효과적영향。방법장60지자성BALB/c소서수궤분위대조조、니감다당조、역묘조、역묘+좌제조등4조，매조15지，분별피하주사100μl PBS、100μg니감다당、100μg pcD?NA3.1?Sj26GST혈흡충DNA역묘、100μg pcDNA3.1?Sj26GST여등량니감다당좌제적혼합물。각조분별우실험개시시、제2주、제4주각면역1차。말차면역후제2주，이일본혈흡충미유40±1조/지경복부피부공격감염소서。감염후6주부살소서，수집혈청、간장급성충，이ELISA법측정혈청중특이성IgG수평，계산감충솔급매극간조직감란솔。결과감염후6주，역묘조화역묘+좌제조소서혈청중IgG항체수평분별위18.26±0.42 mg/ml화20.21±0.89 mg/ml，차이유통계학의의（P<0.05），량조균현저고우대조조（P균<0.05）；역묘조감충솔화감란솔분별위28.60%화35.84%，역묘+좌제조감충솔화감란솔분별위38.04%화49.74%，차이균유통계학의의（P균<0.05），차균현저고우PBS대조조（P균<0.01）。결론니감다당작위좌제，대일본혈흡충DNA역묘구유명현증효작용。
Objective To discuss the effect of polysaccharides from Tegillarca granosa as an adjuvant on pcDNA3.1?Sj26GST vaccine against Schistosoma japonicum infection. Methods Sixty SPF BALB/c mice were divided into four groups randomly(15 mice each group),including a control group,a polysaccharides group,a vaccine group,and a vaccine plus poly?saccharides group. In the 0,2th and 4th week of the experiment,the mice in the above four groups were immunized for 3 times with 100μl PBS,100μg polysaccharides from Tegillarca granosa,100μg Sj26GST vaccine,and 100μg Sj26GST vaccine plus equivalent polysaccharides,respectively. Two weeks after the last immunization,all the mice were infected with 40 ± 1 Schistosoma japonicum cercariae through the skin of the abdomen. After the infection for 6 weeks,all the mice were sacrificed, and their serums,livers and the adult worm of Schistosoma japonicum in them were collected,the specific sera IgG antibodies were detected by ELISA,and the worm reduction rates and egg reduction rates in the liver were calculated. Results Six weeks after the infection,the IgG antibody levels of the mice in the vaccine group and the vaccine plus polysaccharides group were 18.26 ± 0.42 mg/ml and 20.21 ± 0.89 mg/ml respectively,the difference between them were statistically significant,and both of the IgG levels of the above groups were significantly higher than those in the control(both P<0.05). The worm reduction rate and egg reduction rate in the vaccine group were 28.60%and 35.84%,respectively,and the rates in the vaccine plus polysac?charides group were 38.04% and 49.74%,respectively,the differences between the worm reduction rates and egg reduction rates were both statistically significant(both P<0.05). Meanwhile,the two rates in the two above groups were all significantly higher than those in the control group(all P<0.01). Conclusion Polysaccharides from Tegillarca granosa using as an adju?vant can increase the protection effect of pcDNA3.1?Sj26GST vaccine against Schistosoma japonicum infection.
