中国血吸虫病防治杂志
中國血吸蟲病防治雜誌
중국혈흡충병방치잡지
CHINESE JOURNAL OF SCHISTOSOMIASIS CONTROL
2014年
6期
618-621
,共4页
张瑕%崔彩霞%张维荣%晓红%马念%邹萍%涂祖武%胡合华%刘雄%曹淳力%许静
張瑕%崔綵霞%張維榮%曉紅%馬唸%鄒萍%塗祖武%鬍閤華%劉雄%曹淳力%許靜
장하%최채하%장유영%효홍%마념%추평%도조무%호합화%류웅%조순력%허정
日本血吸虫病%病原学检测%免疫学检测%查病模式%效能%湖沼型地区
日本血吸蟲病%病原學檢測%免疫學檢測%查病模式%效能%湖沼型地區
일본혈흡충병%병원학검측%면역학검측%사병모식%효능%호소형지구
Schistosomiasis japonica%Etiologic detection%Immunological detection%Detection pattern%Efficacy%Lake and marshland area
目的：评价湖沼型流行区常用血吸虫病查病模式效能。方法选择江陵县荆干村作为调查点，采集6～65岁常住居民静脉血，采用间接血凝试验（IHA）、胶体染料试纸条法（DDIA）进行平行检测，对任一方法检测阳性者采用Kato?Katz法、尼龙绢集卵孵化法进行平行检测。对两种免疫学方法的一致性进行评价，并分别以IHA、DDIA、IHA+DDIA法筛查再加病原学检测3种查病模式来推算受检人群的感染率。结果血清学方法共检测530人，DDIA、IHA的抗体阳性率分别为46.98%（249/530）、28.49%（151/530），前者显著高于后者（χ2=59.55，P<0.01）。两种方法共检出抗体阳性者279人，其中252人接受了病原学检测，Kato?Katz法和集卵孵化法共检出血吸虫感染者22例，IHA、DDIA分别漏检7例和3例。IHA、DDIA、IHA+DDIA法筛查再加病原学检测3种查病模式对530例受检人群的推算感染率分别是3.14%、3.97%、4.60%。结论在疫情持续降低的情况下，当前常用的血吸虫病查病模式易导致漏诊，应开发更敏感和有效的诊断方法，并探索适合低度流行态势下的血吸虫病筛查新模式。
目的：評價湖沼型流行區常用血吸蟲病查病模式效能。方法選擇江陵縣荊榦村作為調查點，採集6～65歲常住居民靜脈血，採用間接血凝試驗（IHA）、膠體染料試紙條法（DDIA）進行平行檢測，對任一方法檢測暘性者採用Kato?Katz法、尼龍絹集卵孵化法進行平行檢測。對兩種免疫學方法的一緻性進行評價，併分彆以IHA、DDIA、IHA+DDIA法篩查再加病原學檢測3種查病模式來推算受檢人群的感染率。結果血清學方法共檢測530人，DDIA、IHA的抗體暘性率分彆為46.98%（249/530）、28.49%（151/530），前者顯著高于後者（χ2=59.55，P<0.01）。兩種方法共檢齣抗體暘性者279人，其中252人接受瞭病原學檢測，Kato?Katz法和集卵孵化法共檢齣血吸蟲感染者22例，IHA、DDIA分彆漏檢7例和3例。IHA、DDIA、IHA+DDIA法篩查再加病原學檢測3種查病模式對530例受檢人群的推算感染率分彆是3.14%、3.97%、4.60%。結論在疫情持續降低的情況下，噹前常用的血吸蟲病查病模式易導緻漏診，應開髮更敏感和有效的診斷方法，併探索適閤低度流行態勢下的血吸蟲病篩查新模式。
목적：평개호소형류행구상용혈흡충병사병모식효능。방법선택강릉현형간촌작위조사점，채집6～65세상주거민정맥혈，채용간접혈응시험（IHA）、효체염료시지조법（DDIA）진행평행검측，대임일방법검측양성자채용Kato?Katz법、니룡견집란부화법진행평행검측。대량충면역학방법적일치성진행평개，병분별이IHA、DDIA、IHA+DDIA법사사재가병원학검측3충사병모식래추산수검인군적감염솔。결과혈청학방법공검측530인，DDIA、IHA적항체양성솔분별위46.98%（249/530）、28.49%（151/530），전자현저고우후자（χ2=59.55，P<0.01）。량충방법공검출항체양성자279인，기중252인접수료병원학검측，Kato?Katz법화집란부화법공검출혈흡충감염자22례，IHA、DDIA분별루검7례화3례。IHA、DDIA、IHA+DDIA법사사재가병원학검측3충사병모식대530례수검인군적추산감염솔분별시3.14%、3.97%、4.60%。결론재역정지속강저적정황하，당전상용적혈흡충병사병모식역도치루진，응개발경민감화유효적진단방법，병탐색괄합저도류행태세하적혈흡충병사사신모식。
Objective To evaluate the efficacy of routinely used pattern for schistosomiasis diagnosis in lake and marshland regions. Methods A historically heavy endemic village of schistosomiasis named Jinggan Village from Jiangling County was se?lected for field survey. The residents aged 6?65 years were screened by indirect hemagglutination assay(IHA)and dipstick dye immunoassay(DDIA)in parallel. The serological positives were examined by Kato?Katz technique and miracidium hatching technique to determine the infection of schistosome. The consistency of the two serological methods was evaluated. In addition , the schistosome infection rates were estimated according to the 3 detection patterns namely IHA,DDIA,IHA+DDIA combined with the etiologic examination. Results A total of 530 individuals were examined by the serological tests. The positive rate of DDIA was 46.98%(249/530),significantly higher than that of IHA(28.49%,151/530)(χ2=59.55,P<0.01). Totally 279 in?dividuals were serological positives determined by IHA or DDIA,while 252 of them were detected by stool examination,and 22 cases were determined as parasitological positives,while 7 and 3 cases were diagnosed as antibody negatives by IHA and DDIA,respectively. The estimated infection rates determined by IHA,DDIA,IHA plus DDIA combined with stool examination were 3.14%,3.97%、4.60%,respectively. Conclusions Under the condition of endemic situation becoming more and more waning,the current routinely used pattern for schistosomiasis detection may lead to missed diagnosis. So,more sensitive and ef?fective diagnostic tools or appropriate detection patterns need to be explored.
