CAJ | 학술논문

采用溶剂提取和两次硅胶柱层析法分离鸦胆子油中的β-谷甾醇，并用波谱学方法进行鉴定；对鸦胆子油用氢氧化钾－乙醇皂化后，采用气相色谱法对β-谷甾醇的含量进行测定．色谱条件为：Agilent HP-5毛细管柱（30 m ×250μm ×0.25μm）；FID 检测器；进样口温度为285℃；程序升温：270℃（24 min），10℃·min －1升温至280℃（15 min）；检测器温度为300℃；分流比为40∶1；流速为1 mL·min －1；进样量为1μL．β-谷甾醇对照品的峰面积与质量浓度在0.02847～0.4100 mg·mL －1呈良好的线性关系（r ＝0.9994）．该方法的平均回收率为95.2％（RSD ＝2.98％），测得8份样品中β-谷甾醇的质量分数在2.96～4.37 mg·g －1．本方法快速、简便、重复性好，可用于鸦胆子油中β-谷甾醇的含量测定．
채용용제제취화량차규효주층석법분리아담자유중적β-곡치순，병용파보학방법진행감정；대아담자유용경양화갑－을순조화후，채용기상색보법대β-곡치순적함량진행측정．색보조건위：Agilent HP-5모세관주（30 m ×250μm ×0.25μm）；FID 검측기；진양구온도위285℃；정서승온：270℃（24 min），10℃·min －1승온지280℃（15 min）；검측기온도위300℃；분류비위40∶1；류속위1 mL·min －1；진양량위1μL．β-곡치순대조품적봉면적여질량농도재0.02847～0.4100 mg·mL －1정량호적선성관계（r ＝0.9994）．해방법적평균회수솔위95.2％（RSD ＝2.98％），측득8빈양품중β-곡치순적질량분수재2.96～4.37 mg·g －1．본방법쾌속、간편、중복성호，가용우아담자유중β-곡치순적함량측정．
The β-sitosterol was isolated by solvent extraction and silica gel column chromatography (two times),and identified by spectroscopic methods.A gas chromatography (GC)method was estab-lished for the determination of β-sitosterol in 8 batches of Bruceae Fructus oil.The oil samples of Bruceae Fructus from 8 different areas were analyzed by a Agilent HP-5 capillary column after saponification with KOH-EtOH.The temperature was maintained at 270 ℃ for 24 min,then increased to 280 ℃ at a rate of 10 ℃ ·min -1 and was maintained for 15 min.The injection temperature was 285 ℃ and the detection temperature was 300 ℃.Carrier gas was nitrogen,flow rate was 1.01 mL·min -1 with the spilt 40∶1. The linear range of β-sitosterol was 0.028 47 -0.410 0 mg·mL -1 (r =0.999 4).The average recovery was 95.2% (RSD =2.98%),and the content of β-sitosterol in 8 batches of Bruceae Fructus oil was in the range of 2.96 -4.37 mg·g -1 .The method for determining the content of β-sitosterol was accurate with good reproducibility,and could be used for the determination of β-sitosterol in Bruceae Fructus oil.