现代农业科技
現代農業科技
현대농업과기
XIANDAIHUA NONGYE
2014年
22期
136-137,139
,共3页
黄敏%梁春辉%岳海林%李荣喜%郭晓珍%王丽萍%李秀平
黃敏%樑春輝%嶽海林%李榮喜%郭曉珍%王麗萍%李秀平
황민%량춘휘%악해림%리영희%곽효진%왕려평%리수평
Sorbonne%鳞片%离体培养%组织培养
Sorbonne%鱗片%離體培養%組織培養
Sorbonne%린편%리체배양%조직배양
Sorbonne%bulb scale%culture in vitro%tissue culture
以东方百合品种‘Sorbonne’鳞片为外植体,研究不同因子对‘Sorbonne’消毒、诱导、增殖、生根等的影响。结果表明:‘Sorbonne’外层鳞片消毒最为困难,而中层和内层差异不大;最佳消毒方法是外层鳞片用75%酒精处理30 s再用0.1%升汞浸泡18~20 min;中层和内层鳞片用75%酒精处理30 s,再用0.1%升汞浸泡14~16 min,其中不同部位鳞片诱导率从高到低分别为外层跃中层跃内层,其中外层的诱导率为76.7%;百合鳞片最佳增殖培养基为MS+6-BA 2.0 mg/L+NAA 0.2 mg/L,增殖系数为6.8;最佳生根培养基为1/2MS+6-BA 0.5 mg/L+NAA 0.2 mg/L,平均生根数为20条。
以東方百閤品種‘Sorbonne’鱗片為外植體,研究不同因子對‘Sorbonne’消毒、誘導、增殖、生根等的影響。結果錶明:‘Sorbonne’外層鱗片消毒最為睏難,而中層和內層差異不大;最佳消毒方法是外層鱗片用75%酒精處理30 s再用0.1%升汞浸泡18~20 min;中層和內層鱗片用75%酒精處理30 s,再用0.1%升汞浸泡14~16 min,其中不同部位鱗片誘導率從高到低分彆為外層躍中層躍內層,其中外層的誘導率為76.7%;百閤鱗片最佳增殖培養基為MS+6-BA 2.0 mg/L+NAA 0.2 mg/L,增殖繫數為6.8;最佳生根培養基為1/2MS+6-BA 0.5 mg/L+NAA 0.2 mg/L,平均生根數為20條。
이동방백합품충‘Sorbonne’린편위외식체,연구불동인자대‘Sorbonne’소독、유도、증식、생근등적영향。결과표명:‘Sorbonne’외층린편소독최위곤난,이중층화내층차이불대;최가소독방법시외층린편용75%주정처리30 s재용0.1%승홍침포18~20 min;중층화내층린편용75%주정처리30 s,재용0.1%승홍침포14~16 min,기중불동부위린편유도솔종고도저분별위외층약중층약내층,기중외층적유도솔위76.7%;백합린편최가증식배양기위MS+6-BA 2.0 mg/L+NAA 0.2 mg/L,증식계수위6.8;최가생근배양기위1/2MS+6-BA 0.5 mg/L+NAA 0.2 mg/L,평균생근수위20조。
The bulb scales of Lilium oriental Hybrids ’Sorbonne’ were used as explants for in vitro culture. The results showed that the best disinfection method of the outer bulb scale explants of ’Sorbonne’ was 75% ethanol for 30 s,followed by 0.1% HgCl2 18~20 min,and the best disinfection method of the middle scale and the inner scale bulb scale explants of’Sorbonne’was 75%ethanol for 30 s,followed by 0.1%HgCl2 14~16 min. The most difficult was the outer scale disinfection,but difference of disinfection effect of the middle scale and the inner scale was smaller. The scales in different parts had different rate of induction of bulbs as followsthe outer scales>the middle scales>the inner scales. The induction rate of the outer scales was 76.7%. The appropriate medium for multiplication was MS+6-BA 2.0 mg/L+NAA 0.2 mg/L with the multiplication coefficient of 6.8;the best medium for root induction was 1/2MS+6-BA 0.5 mg/L+NAA 0.2 mg/L and the number of roots per plantlets of 20.