CAJ | 학술논문

目的：对江西南昌郊区松树花粉的变应原组分进行分离及免疫学鉴定。方法提取松树花粉粗提液，然后通过十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)对松树花粉的蛋白质组分进行分离并测定其分子量，采用免疫印迹(West-ern-blotting)法鉴定其变应原成分。结果松树花粉粗提液有10余条蛋白带，其中分子量为12kD、26kD和100kD的蛋白可与松树花粉过敏性病人血清IgE 结合，其中26kD为主要变应原。结论对松树花粉变应原进行了初步的分离、鉴定，为进一步对松树花粉变态反应性疾病的临床诊断和治疗提供初步的实验基础。
목적：대강서남창교구송수화분적변응원조분진행분리급면역학감정。방법제취송수화분조제액，연후통과십이완기광산납-취병희선알응효전영(SDS-PAGE)대송수화분적단백질조분진행분리병측정기분자량，채용면역인적(West-ern-blotting)법감정기변응원성분。결과송수화분조제액유10여조단백대，기중분자량위12kD、26kD화100kD적단백가여송수화분과민성병인혈청IgE 결합，기중26kD위주요변응원。결론대송수화분변응원진행료초보적분리、감정，위진일보대송수화분변태반응성질병적림상진단화치료제공초보적실험기출。
Objective To isolate and characterize the allergenic components of pine pollen. Methods Pine pollen coarse ex-traction fluid was extracted, and the proteins of pine pollen extraction and their molecular were analyzed by SDS-PAGE. To char-acterize the allergenic components, pine pollen coarse extraction fluid was analyzed by western-blotting. Results The pine pollen coarse extraction fluid displayed more than ten protein bands by SDS-PAGE,and three bands (relative molecular weights were 12 kD, 26 kD and 100 kD, respectively) could react with IgE in the sera of patients with allergy to pine pollen. Among the three bands, the 26 kD protein was the major allergen. Conclusion Our study of preliminary isolation and immunological characteriza-tion of the allergenic components in pine pollen will be used as a base for diagnosis and therapy of pine pollen related allergy.