CAJ | 학술논문

目的：分析江西省2009-2012年甲型H3N2流感病毒M2以及NA基因的特点，掌握其耐药情况，为临床治疗和疾病控制提供参考。方法从江西省流感监测网中随机选择19株甲型H3N2流感病毒，经核酸提取和one-step RT-PCR扩增M以及NA基因片段，双向序列测定，采用DNAStar5.0和 Mage4.0序列分析软件分析M2以及NA基因特征以及耐药性位点。结果19株毒株M2基因31位氨基酸均由丝氨酸(S)突变为天冬酰胺(N)；所有毒株NA蛋白催化活性位点和辅助位点均未发生氨基酸替换。结论19株毒株均对金刚烷胺类药物耐药，对流感病毒神经氨酸酶抑制剂药物均敏感，但仍应加强对流感病毒的耐药性监测。
목적：분석강서성2009-2012년갑형H3N2류감병독M2이급NA기인적특점，장악기내약정황，위림상치료화질병공제제공삼고。방법종강서성류감감측망중수궤선택19주갑형H3N2류감병독，경핵산제취화one-step RT-PCR확증M이급NA기인편단，쌍향서렬측정，채용DNAStar5.0화 Mage4.0서렬분석연건분석M2이급NA기인특정이급내약성위점。결과19주독주M2기인31위안기산균유사안산(S)돌변위천동선알(N)；소유독주NA단백최화활성위점화보조위점균미발생안기산체환。결론19주독주균대금강완알류약물내약，대류감병독신경안산매억제제약물균민감，단잉응가강대류감병독적내약성감측。
Objective To analyze the characteristics of M2 and NA genes of H3N2 influenza virus in Jiangxi provience dur-ing 2009-2012. Methods Nineteen strains of H3N2 influenza virus were randomly selected for detection and virus RNA were ex-tracted. Fragments of M2 and NA genes were amplified by one-step RT-PCR and then were sequenced. The data obtained were analyzed with the software DNAStar5.0 and Mage4.0. Results All strains of H3N2 influenza virus had amino acid changed at site S31N in M2 gene. All strains of H3N2 influenza virus had no mutation in catalytic residues and framework residues of NA gene. Conclusions The 19 strains were resistant to amantadine, and sensitive to neuraminidase inhibitors, however, continuous resistance surveillance is also necessary for control and prevention of influenza.