实验与检验医学
實驗與檢驗醫學
실험여검험의학
EXPERIMENTAL AND LABORATORY MEDICINE
2014年
6期
654-657
,共4页
人宫颈癌基因%原发性肝细胞癌%肝硬化%mRNA
人宮頸癌基因%原髮性肝細胞癌%肝硬化%mRNA
인궁경암기인%원발성간세포암%간경화%mRNA
Human cervical cancer oncogene%Primary hepatocellular carcinoma%Hepatocirrhosis%mRNA
目的:探讨人宫颈癌基因(human cervical cancer oncogene,HCCR) mRNA在原发性肝细胞癌(primary hepatocellular carcinoma,PHC)患者中的表达及其临床意义。方法采用RT-PCR检测PHC癌组织、癌旁肝组织和肝硬化组织及各组外周血单个核细胞(peripheral blood mononuclear cell,PBMC)中的HCCR mRNA表达水平,并进行分析。结果 HCCR mRNA 在PHC组织中的相对表达强度为0.83±0.10,癌旁肝组织中的相对表达强度为0.12±0.07,肝硬化组织中的相对表达强度为0.57±0.12,三组均数间两两比较,差异均有统计学意义(P<0.05)。 PBMC中,肝癌患者HCCR mRNA的表达强度为0.55±0.06,肝硬化患者HCCR mRNA的表达强度为0.34±0.04,而正常人未检测出,差异均有统计学意义(P<0.05)。结论 PHC和肝硬化患者的组织和PBMC中HCCR表达明显增高,HCCR基因可能参与了PHC的发生与发展,其与PHC的恶变演进有一定的相关性。
目的:探討人宮頸癌基因(human cervical cancer oncogene,HCCR) mRNA在原髮性肝細胞癌(primary hepatocellular carcinoma,PHC)患者中的錶達及其臨床意義。方法採用RT-PCR檢測PHC癌組織、癌徬肝組織和肝硬化組織及各組外週血單箇覈細胞(peripheral blood mononuclear cell,PBMC)中的HCCR mRNA錶達水平,併進行分析。結果 HCCR mRNA 在PHC組織中的相對錶達彊度為0.83±0.10,癌徬肝組織中的相對錶達彊度為0.12±0.07,肝硬化組織中的相對錶達彊度為0.57±0.12,三組均數間兩兩比較,差異均有統計學意義(P<0.05)。 PBMC中,肝癌患者HCCR mRNA的錶達彊度為0.55±0.06,肝硬化患者HCCR mRNA的錶達彊度為0.34±0.04,而正常人未檢測齣,差異均有統計學意義(P<0.05)。結論 PHC和肝硬化患者的組織和PBMC中HCCR錶達明顯增高,HCCR基因可能參與瞭PHC的髮生與髮展,其與PHC的噁變縯進有一定的相關性。
목적:탐토인궁경암기인(human cervical cancer oncogene,HCCR) mRNA재원발성간세포암(primary hepatocellular carcinoma,PHC)환자중적표체급기림상의의。방법채용RT-PCR검측PHC암조직、암방간조직화간경화조직급각조외주혈단개핵세포(peripheral blood mononuclear cell,PBMC)중적HCCR mRNA표체수평,병진행분석。결과 HCCR mRNA 재PHC조직중적상대표체강도위0.83±0.10,암방간조직중적상대표체강도위0.12±0.07,간경화조직중적상대표체강도위0.57±0.12,삼조균수간량량비교,차이균유통계학의의(P<0.05)。 PBMC중,간암환자HCCR mRNA적표체강도위0.55±0.06,간경화환자HCCR mRNA적표체강도위0.34±0.04,이정상인미검측출,차이균유통계학의의(P<0.05)。결론 PHC화간경화환자적조직화PBMC중HCCR표체명현증고,HCCR기인가능삼여료PHC적발생여발전,기여PHC적악변연진유일정적상관성。
Objective To detect the expression level of HCCR mRNA in primary hepatocellular carcinoma(PHC) and explore its clinical significance. Methods Reverse transcription polymerase chain reaction was used to detect the expression of HCCR mRNA in PHC tissues, surrounding non-tumor tissues, hepatocirrhosis tissues and peripheral blood mononuclear cells. Results The relative expression level of HCCR mRNA in PHC tissues was 0.83±0.10, and which in surrounding non-tumor tissues and the hepatocirrhosis tissues were 0.12±0.07 and 0.57±0.12, respectively. The differences in the three groups had statistical significance (P<0.05). The relative mRNA level of HCCR in the PBMC of PHC patients were 0.55 ±0.06 and which in the hepatocirrhosis pa-tients were 0.34±0.04, while HCCR expression in the normal ones could not detected. The differences had statistical significance (P<0.05). Conclusion The mRNA levels of HCCR in PHC tissues, hepatocirrhosis tissues and their PBMC are up-regulated sig-nificantly, which suggests that HCCR maybe involved in the occurrence and development of PHC, and maybe related to the ma-lignant change of PHC.