中国康复理论与实践
中國康複理論與實踐
중국강복이론여실천
CHINESE JOURNAL OF REHABILITATION THEORY & PRACTICE
2014年
12期
1175-1178
,共4页
朱森%黄迅悟%梁艳%彭伟%冯会成%张瑞斐
硃森%黃迅悟%樑豔%彭偉%馮會成%張瑞斐
주삼%황신오%량염%팽위%풍회성%장서비
关节结核%γ-干扰素%诊断%结核分枝杆菌%特异性蛋白
關節結覈%γ-榦擾素%診斷%結覈分枝桿菌%特異性蛋白
관절결핵%γ-간우소%진단%결핵분지간균%특이성단백
joint tuberculosis%interferon-gamma%diagnosis%mycobacterium tuberculosis%specific proteins
目的：探讨全血γ-干扰素(IFN-γ)检测在活动性关节结核诊断的意义。方法以5种结核分枝杆菌特异性蛋白作为刺激蛋白，应用化学发光酶免疫分吸附法检测35例活动性关节结核病患者及35名健康体检者的全血IFN-γ含量，比较分析5种蛋白刺激时对活动性关节结核检测的敏感性、特异性、阳性预测值、阴性预测值、阳性似然比和阴性似然比，评估其诊断效能。结果CFP10/ESAT6融合蛋白、Rv2041c蛋白、Rv0057/1352融合蛋白、Rv1419蛋白及Rv1656蛋白刺激时，全血IFN-γ检测灵敏性为77.1%、68.6%、71.4%、74.3%和65.7%，特异性为65.7%、54.3%、57.1%、60.0%和62.9%，阳性预测值为62.9%、60%、62.5%、65%及63.9%，阴性预测值为74.2%、63.3%、66.7%、70%及64.7%，阳性似然比为2.25、1.5、1.67、1.86及1.77，阴性似然比为0.348、0.579、0.5、0.429及0.545。结论全血IFN-γ检测对关节结核有一定参考意义，但不能作为活动性关节结核的确诊指标。
目的：探討全血γ-榦擾素(IFN-γ)檢測在活動性關節結覈診斷的意義。方法以5種結覈分枝桿菌特異性蛋白作為刺激蛋白，應用化學髮光酶免疫分吸附法檢測35例活動性關節結覈病患者及35名健康體檢者的全血IFN-γ含量，比較分析5種蛋白刺激時對活動性關節結覈檢測的敏感性、特異性、暘性預測值、陰性預測值、暘性似然比和陰性似然比，評估其診斷效能。結果CFP10/ESAT6融閤蛋白、Rv2041c蛋白、Rv0057/1352融閤蛋白、Rv1419蛋白及Rv1656蛋白刺激時，全血IFN-γ檢測靈敏性為77.1%、68.6%、71.4%、74.3%和65.7%，特異性為65.7%、54.3%、57.1%、60.0%和62.9%，暘性預測值為62.9%、60%、62.5%、65%及63.9%，陰性預測值為74.2%、63.3%、66.7%、70%及64.7%，暘性似然比為2.25、1.5、1.67、1.86及1.77，陰性似然比為0.348、0.579、0.5、0.429及0.545。結論全血IFN-γ檢測對關節結覈有一定參攷意義，但不能作為活動性關節結覈的確診指標。
목적：탐토전혈γ-간우소(IFN-γ)검측재활동성관절결핵진단적의의。방법이5충결핵분지간균특이성단백작위자격단백，응용화학발광매면역분흡부법검측35례활동성관절결핵병환자급35명건강체검자적전혈IFN-γ함량，비교분석5충단백자격시대활동성관절결핵검측적민감성、특이성、양성예측치、음성예측치、양성사연비화음성사연비，평고기진단효능。결과CFP10/ESAT6융합단백、Rv2041c단백、Rv0057/1352융합단백、Rv1419단백급Rv1656단백자격시，전혈IFN-γ검측령민성위77.1%、68.6%、71.4%、74.3%화65.7%，특이성위65.7%、54.3%、57.1%、60.0%화62.9%，양성예측치위62.9%、60%、62.5%、65%급63.9%，음성예측치위74.2%、63.3%、66.7%、70%급64.7%，양성사연비위2.25、1.5、1.67、1.86급1.77，음성사연비위0.348、0.579、0.5、0.429급0.545。결론전혈IFN-γ검측대관절결핵유일정삼고의의，단불능작위활동성관절결핵적학진지표。
Objective To discuss the significance of the detection of interferon-γin the diagnosis of active joint tuberculosis. Methods 5 kinds of specific proteins of mycobacterium tuberculosis were used as stimulating protein, the chemiluminescence enzyme immunoassay was used to test the content of interferon-γ. The interferon-γof 35 cases of patients with active joint tuberculosis and 35 cases of healthy people were detected. The difference of the sensitivity, specificity, positive predictive value, negative predictive value, positive likelihood ra-tio and negative likelihood ratio of 5 kinds of proteins to the diagnostic of active joint tuberculosis were compared. Results CFP10/ESAT6 fusion protein, Rv2041c protein, Rv0057/1352 fusion proteins, Rv1419 protein and Rv1656 protein were used as stimulating protein, the sensitivities of active joint tuberculosis by the whole blood interferon-γassay were 77.1%, 68.6%, 71.4%, 74.3%and 65.7%. The specifici-ties were 65.7%, 54.3%, 57.1%, 60.0%and 62.9%. The positive predictive values were 62.9%, 60%, 62.5%, 65%and 63.9%. The negative predictive values were 74.2%, 63.3%, 66.7%, 70%and 64.7%. The positive likelihood ratios were 2.25, 1.5, 1.67, 1.86 and 1.77. The nega-tive likelihood ratios were 0.348, 0.579, 0.5, 0.429 and 0.545. Conclusion The detection of interferon-γhas certain significance to joint tu-berculosis. But it is not as an index of diagnosing joint tuberculosis.
