CAJ | 학술논문

目的：比较腺相关病毒(AAV)载体和慢病毒(LV)载体在间充质干细胞(MSCs)基因转染中的效率。方法密度梯度离心法分离培养MSCs， HE染色、Nestin免疫荧光染色鉴定， BrdU标记观察增殖情况。分别包装AAV与LV假病毒颗粒，并感染MSCs，通过β-gal染色和绿色荧光蛋白检测，分别计算两者的转染效率。结果 MSCs成功从骨髓分离。AAV载体介导的基因转染率为49.1%，LV载体的转染率为91.4%(P<0.01)。结论 LV载体介导的基因转染方法转染效率更高。
목적：비교선상관병독(AAV)재체화만병독(LV)재체재간충질간세포(MSCs)기인전염중적효솔。방법밀도제도리심법분리배양MSCs， HE염색、Nestin면역형광염색감정， BrdU표기관찰증식정황。분별포장AAV여LV가병독과립，병감염MSCs，통과β-gal염색화록색형광단백검측，분별계산량자적전염효솔。결과 MSCs성공종골수분리。AAV재체개도적기인전염솔위49.1%，LV재체적전염솔위91.4%(P<0.01)。결론 LV재체개도적기인전염방법전염효솔경고。
Objective To compare 2 kinds of commonly used viral vectors, adeno-associated viral (AAV) vector and lentiviral (LV) vec-tor in the gene transfection for bone marrow derived mesenchymal stem cells (MSCs). Methods MSCs were isolated with density gradient (lymphocytes seperation) and identified with HE staining and immunocytochemistory staining for Nestin. The proliferation of BMSCs was detected with BrdU labeling. AAV mediated gene transfection was carried out through recombinant AAV-LacZ viral particles. For LV medi-ated gene transfection, the LV particles were used directly. The transfection efficiency was estimated withβ-gal staining and green fluores-cent protein under the fluorescent microscope respectively. Results MSCs was successfully isolated from the bone marrow. HE staining showed that MSCs was with big nucleus, 1-3 nucleoli, and high nucleocytoplasmic ratio. BrdU labeling suggested that MSCs were prolifer-ating. Some MSCs expressed Nestin. The gene transfection efficiency mediated with AAV vector was 49.1%, and it was 91.4%with LV vec-tor (P<0.01). Conclusion The LV vector is more efficient on gene transfection than AAV vector.