CAJ | 학술논문

该研究首次利用功能单体2-乙烯基-4,6-二氨基-1,3,5-三嗪(VDAT)在水相中合成具有生物应用前景的微米级核壳微球。首先,我们利用分散聚合制备出了微米级,单分散,且表面带有足够负电荷的聚苯乙烯微球(PS)模板。再利用半连续无皂乳液聚合法在阳离子引发剂的作用下,于水相中将微溶于热水的单体VDAT引发聚合。通过静电作用将带正电荷的VDAT聚合物(PVDAT)包覆于带负电荷的 PS 微球表面,成功合成PVDAT＠PS 功能微球。最后利用该功能微球对不同双链 DNA 的吸附,初步研究其在生物领域的应用。
해연구수차이용공능단체2-을희기-4,6-이안기-1,3,5-삼진(VDAT)재수상중합성구유생물응용전경적미미급핵각미구。수선,아문이용분산취합제비출료미미급,단분산,차표면대유족구부전하적취분을희미구(PS)모판。재이용반련속무조유액취합법재양리자인발제적작용하,우수상중장미용우열수적단체VDAT인발취합。통과정전작용장대정전하적VDAT취합물(PVDAT)포복우대부전하적 PS 미구표면,성공합성PVDAT＠PS 공능미구。최후이용해공능미구대불동쌍련 DNA 적흡부,초보연구기재생물영역적응용。
This research is the first one to synthesize micron grade core-shell microspheres with functional mon-omer 2-vinyl-4,6-diamino-1,3,5-triazine(VDAT)for biological application in water.The results showed that, the micron grade,monodisperse,and negatively charged polystyrene microspheres(PS)have been prepared by dispersion polymerization.And the monomer VDAT which is slightly soluble in hot aqueous phase has been pol-ymerized with cationic initiator in water through semi-continuous soap-free emulsion polymerization.Then due to the electrostatic effect,the positively charged poly(VDAT)coated on the negatively charged PS surface and formed the PVDAT@PS microspheres.Finally,the great value of PVDAT@PS functional microspheres in the field of biology has been preliminarily studied through the adsorption situation of PVDAT@PS and different ds-DNA.