CAJ | 학술논문

本文研究嗜水气单胞菌（Aeromonas hydrophila，Ah）感染对1+龄施氏鲟（Acipenser schrencki）血清中补体、一氧化氮（NO）水平及一氧化氮合酶（NOS）活性的影响，以探讨鲟科鱼类细菌感染应答的敏感因子。首先采用腹腔注射法测定了Ah对施氏鲟的LD50，然后给健康非免疫状态的1+龄施氏鲟，注射LD50剂量Ah菌液，观察发病情况，同时采集健康组和病理模型组血清及肝组织样品，测定血清中补体、NO水平及NOS活性。结果显示：施氏鲟在感染Ah时，血清中补体C3、C4的含量均有一定程度升高，其中C3的含量显著升高，表明C3在施氏鲟对抗细菌感染中起重要作用。NO是感染应答中重要的炎性反应调节分子，在Ah感染后，施氏鲟血清中NO的水平显著升高，肝脏中的NO水平变化不显著。NOS在NO的生成中起关键作用，但施氏鲟感染后血清及肝脏中的NOS活性无显著变化，由于未做分型检测，属正常现象。
본문연구기수기단포균（Aeromonas hydrophila，Ah）감염대1+령시씨심（Acipenser schrencki）혈청중보체、일양화담（NO）수평급일양화담합매（NOS）활성적영향，이탐토심과어류세균감염응답적민감인자。수선채용복강주사법측정료Ah대시씨심적LD50，연후급건강비면역상태적1+령시씨심，주사LD50제량Ah균액，관찰발병정황，동시채집건강조화병리모형조혈청급간조직양품，측정혈청중보체、NO수평급NOS활성。결과현시：시씨심재감염Ah시，혈청중보체C3、C4적함량균유일정정도승고，기중C3적함량현저승고，표명C3재시씨심대항세균감염중기중요작용。NO시감염응답중중요적염성반응조절분자，재Ah감염후，시씨심혈청중NO적수평현저승고，간장중적NO수평변화불현저。NOS재NO적생성중기관건작용，단시씨심감염후혈청급간장중적NOS활성무현저변화，유우미주분형검측，속정상현상。
The C3, C4 and nitric oxide (NO) levels and NO synthetase (NOS) activity were determined in blood and liver of 1+year old Amur sturgeon Acipenser schrencki intraperitoneally injected with bacterium Aeromonas hydrophila (Ah) at a dose of LD50 in order to find out the sensitive factors in response to bacterial infection. The C3 and C4 contents in serum were found to be increased in the fish challenged with Ah, significant difference in C3 content, indicating that C3 played an important role in Ah infection in Amur sturgeon. The content of NO as an important molecule in immune infectious response was found to be increased significantly in serum of the A-mur sturgeon with Ah infection compared to the normal fish. In liver, however, there was no significant difference in NO content be-tween the Amur sturgeon infected by Ah and the normal fish. There were no significant differences in NOS activity in serum and liver between the normal fish and the fish infected, though the NOS took a key role in NO synthesis.