CAJ | 학술논문

目的：探讨结直肠癌组织中MAGE基因启动子去甲基化状态与其mRNA表达的关系。方法分别用RT-PCR法和MSP法检测65例结直肠癌及相邻正常黏膜组织中MAGE-1、MAGE-3基因mRNA表达、去甲基化状态，并分析二者的关系。结果65例结直肠癌组织中MAGE-1、MAGE-3 mRNA表达率分别为18．5％（12／65）、33．9％（22／65），相邻正常黏膜组织未见表达；其启动子去甲基化率分别为29．2％（19／65）、47．7％（31／65），相邻正常黏膜组织分别为1．5％（1／65）和4．6％（3／65）。 MAGE-1、MAGE-3基因启动子去甲基化与其mRNA表达均显著相关（P均＜0．05）。结论 MAGE-1、MAGE-3基因启动子去甲基化是结直肠癌发生的一个早期、频发事件；检测其去甲基化状态可能有助于结直肠癌的早期诊断和预后判断。
목적：탐토결직장암조직중MAGE기인계동자거갑기화상태여기mRNA표체적관계。방법분별용RT-PCR법화MSP법검측65례결직장암급상린정상점막조직중MAGE-1、MAGE-3기인mRNA표체、거갑기화상태，병분석이자적관계。결과65례결직장암조직중MAGE-1、MAGE-3 mRNA표체솔분별위18．5％（12／65）、33．9％（22／65），상린정상점막조직미견표체；기계동자거갑기화솔분별위29．2％（19／65）、47．7％（31／65），상린정상점막조직분별위1．5％（1／65）화4．6％（3／65）。 MAGE-1、MAGE-3기인계동자거갑기화여기mRNA표체균현저상관（P균＜0．05）。결론 MAGE-1、MAGE-3기인계동자거갑기화시결직장암발생적일개조기、빈발사건；검측기거갑기화상태가능유조우결직장암적조기진단화예후판단。
Objective To observe the relation between MAGE gene promoter region demethylation and MAGE mRNA expression in colorectal carcinoma .Methods The expression and demethylation of MAGE-1, -3 genes from 65 samples with human colorectal carcinomas and corresponding adjacent colonic mucosa specimens were detected by reverse transcrip -tion polymerase chain reaction(RT-PCR) and methylation-specific PCR.The association between the expression and pro-moter region demethylation of the genes was analyzed .Results Expression rate of MAGE-1, -3 genes was 18.5%(12/65) and 33.9%(22/65), respectively, however, none of the corresponding adjacent non-tumorous tissues expressed any MAGE mRNA.But the positive rates of promoter demethylation of the two genes were as follows : 29.2%(19/65) and 47.7%(31/65) in cancerous tissues , the corresponding adjacent non-tumorous 1.5%(1/65) and 4.6%(3/65) respec-tively.MAGE-1,-3 genes mRNA expression had relation with its promoter demethylaton (all P<0.05).Conclusions Demethylaton in promoter regions of MAGE-1,-3 genes may be the critical mechanism of mRNA expression , and may pro-vide information of early diagnosis and prognosis in colorectal cancer patients .