CAJ | 학술논문

背景：间充质干细胞作为种子细胞广泛应用于组织工程和临床治疗，而安全、高效的分选方法是间充质干细胞建系的前提。 　　目的：综述近年来间充质干细胞分选方法的研究进展，比较各方法的优缺点，为安全、高效分离间充质干细胞提供理论依据。 　　方法：第一作者检索 CNKI 数据库(http：//epub.cnki.net/)和 PubMed 数据库(http：//www.ncbi.nlm.nih.gov/pubmed/)的相关文献。中文检索词为“间充质干细胞、分离方法”；英文检索词为“mesenchymal stem cel s (mscs)，isolation methods”。查阅1965至2014年来间充质干细胞分离方法的相关文献，包括综述、临床研究和基础研究，最终纳入52篇文献。 　　结果与结论：①全骨髓贴壁法可快速获得大量间充质干细胞，但需要进一步纯化。②梯度密度离心法选择密度为1.073 g/mL的介质在原代能得到相对较纯的间充质干细胞。③组织消化法适用于脂肪、脐带等组织进行消化分离，选用胶原酶Ⅱ消化效果更佳，但是均存在操作技术要求高的缺陷。④免疫磁珠法适用于研究表达特定细胞表面标记物的某一个间充质干细胞亚群的生物学特性。⑤各种分离方法相结合也是一种理想选择。⑥对于近年来出现的分离新方法，由于案例有限，其可靠性有待进一步研究。
배경：간충질간세포작위충자세포엄범응용우조직공정화림상치료，이안전、고효적분선방법시간충질간세포건계적전제。 　　목적：종술근년래간충질간세포분선방법적연구진전，비교각방법적우결점，위안전、고효분리간충질간세포제공이론의거。 　　방법：제일작자검색 CNKI 수거고(http：//epub.cnki.net/)화 PubMed 수거고(http：//www.ncbi.nlm.nih.gov/pubmed/)적상관문헌。중문검색사위“간충질간세포、분리방법”；영문검색사위“mesenchymal stem cel s (mscs)，isolation methods”。사열1965지2014년래간충질간세포분리방법적상관문헌，포괄종술、림상연구화기출연구，최종납입52편문헌。 　　결과여결론：①전골수첩벽법가쾌속획득대량간충질간세포，단수요진일보순화。②제도밀도리심법선택밀도위1.073 g/mL적개질재원대능득도상대교순적간충질간세포。③조직소화법괄용우지방、제대등조직진행소화분리，선용효원매Ⅱ소화효과경가，단시균존재조작기술요구고적결함。④면역자주법괄용우연구표체특정세포표면표기물적모일개간충질간세포아군적생물학특성。⑤각충분리방법상결합야시일충이상선택。⑥대우근년래출현적분리신방법，유우안례유한，기가고성유대진일보연구。
BACKGROUND:Mesenchymal stem cel s as potential seeded cel s have been widely used in tissue engineering and clinic therapy;thus, the precise, safe, effective isolation of mesenchymal stem cel s is the particular important premise to build culture system. OBJECTIVE:To review the methods of isolating mesenchymal stem cel s and to compare the merit and demerit of different methods, thereby providing theoretical basis for safe and high-effective isolation of mesenchymal stem cel s. METHODS:A computer-based online research of CNKI and PubMed databases was performed to col ect articles, which included reviews, clinical trials and experiments, published between 1965 and 2014 with the key words of“mesenchymal stem cel s (MSCs), isolation methods”in Chinese and English. A total of 52 articles were included according inclusion and exclusion criteria RESULTS AND CONCLUSION:(1) The whole bone marrow culture method can derive a mass of mesenchymal stem cel s, which need to be purified. (2) The density gradient centrifugation method which uses the media with the density of 1.073 g/mL can be used to harvest more purified cel s. (3) The tissue digestion method is suitable for digestion and isolation of adipose tissue and umbilical cord tissue. Type II col agenase digestion is better, but they are both limited by a high demand for operative techniques. (4) Immunomagnetic bead separation is appropriate to study the biological characteristics of a kind of subpopulation of mesenchymal stem cel s which express special surface markers. (5) The combination method is also an optimal way. (6) Some new methods limited by few dates require further studies.