CAJ | 학술논문

背景：胎盘中含有丰富的细胞群，因其中 CD34+细胞含量高而受到越来越多学者的关注，有望成为临床造血干细胞移植治疗血液病及其他恶性疾病的新来源。 　　目的：探索胎盘来源细胞的数量、集落形成能力及其与母体的嵌合程度。 　　方法：采用健康足月剖腹产妇的胎盘，灌注法及组织块消化法收集胎盘中细胞；流式细胞仪检测胎盘及脐血中 CD34+细胞比例；培养细胞集落，定期观察集落形态并计数；序列特异性引物 PCR 扩增技术及序列特异性寡核苷酸探针方法检测胎盘、脐血和母亲外周血的HLA类型；短串联重复PCR方法检测胎盘细胞、脐血细胞和母亲外周血细胞的嵌合情况。 　　结果与结论：胎盘中 CD34+细胞数量明显优于脐血，且胎盘具有体外多种集落形成能力，集落形成良好，同时胎盘提取细胞中含有母体来源成分。提示胎盘中细胞数量及其基本生物学特性使其有潜力成为临床造血干细胞移植的新来源。
배경：태반중함유봉부적세포군，인기중 CD34+세포함량고이수도월래월다학자적관주，유망성위림상조혈간세포이식치료혈액병급기타악성질병적신래원。 　　목적：탐색태반래원세포적수량、집락형성능력급기여모체적감합정도。 　　방법：채용건강족월부복산부적태반，관주법급조직괴소화법수집태반중세포；류식세포의검측태반급제혈중 CD34+세포비례；배양세포집락，정기관찰집락형태병계수；서렬특이성인물 PCR 확증기술급서렬특이성과핵감산탐침방법검측태반、제혈화모친외주혈적HLA류형；단천련중복PCR방법검측태반세포、제혈세포화모친외주혈세포적감합정황。 　　결과여결론：태반중 CD34+세포수량명현우우제혈，차태반구유체외다충집락형성능력，집락형성량호，동시태반제취세포중함유모체래원성분。제시태반중세포수량급기기본생물학특성사기유잠력성위림상조혈간세포이식적신래원。
BACKGROUND:There are abundant cel populations in the placenta that attracts more and more attentions because of high content of CD34+cel s. It is expected to become a new source of hematopoietic stem cel s for the treatment of hematologic diseases and other malignant diseases. OBJECTIVE:To investigate the amount of cel s derived from placenta, their colony forming ability, and their chimerism analysis. METHODS:Five placentas obtained from five healthy ful-term cesarean women were treated with perfusion method and tissue digestion for the cel col ection. Flow cytometry was used to detect the proportion of CD34+cel s in the placenta and cord blood, fol owed by the culture of cel colonies as wel as regular observation of cel morphology and counting. PCR amplification with sequence-specific primers and sequence-specific oligonucleotide probes were used to examine HLA type of placenta, umbilical cord blood, and maternal peripheral blood;Short tandem repeat PCR was used for chimerism analysis. RESULTS AND CONCLUSION:There were more CD34+cel s in the placenta than in the umbilical cord blood. The placenta had good ability to form multiple colonies in vitro, and there were maternal source components in the placenta. It is concluded that the amount of cel s in the placenta and their biological functions exhibit the potential use of placenta as a new source of hematopoietic stem cel s.