CAJ | 학술논문

背景：国内外动物实验已证实超声联合微泡能够显著增强干细胞移植治疗缺血性心血管疾病，但对其作用机制仍不明确 　　目的：通过超声微泡体外作用于细胞，探讨超声联合微泡能够显著增强干细胞移植治疗缺血性心血管疾病的机制。 　　方法：体外分别培养大鼠血管内皮细胞和骨髓间充质干细胞于培养板中，随机各分为对照组、超声组、超声微泡组。对照组不做干预，超声组以频率1 MHz，输出功率1 W/cm2，持续辐照90 s；超声微泡组加入5μL含氟碳气体脂质体超声微泡(约2×1011 L-1)，以同样超声条件作用90 s。 　　结果与结论：超声微泡组血管内皮细胞上清液的血管内皮生长因子和基质细胞衍生因子1表达水平均比对照组显著增高(P<0.05)。超声组血管内皮生长因子表达水平为与对照组无差异；而基质细胞衍生因子1为较对照组明显降低(P<0.01)。骨髓间充质干细胞干预后CXCR4基因表达，超声微泡组、超声组较对照组均明显增强(P<0.01)，但超声微泡组和超声组无统计学差异(P>0.05)。说明超声(1 MHz，1 W/cm2)联合微泡作用90 s能够促进血管内皮细胞分泌细胞因子(血管内皮生长因子和基质细胞衍生因子1)，同时促进骨髓间充质干细胞CXCR4基因表达，是其增强移植干细胞归巢效应的机制之一。
배경：국내외동물실험이증실초성연합미포능구현저증강간세포이식치료결혈성심혈관질병，단대기작용궤제잉불명학 　　목적：통과초성미포체외작용우세포，탐토초성연합미포능구현저증강간세포이식치료결혈성심혈관질병적궤제。 　　방법：체외분별배양대서혈관내피세포화골수간충질간세포우배양판중，수궤각분위대조조、초성조、초성미포조。대조조불주간예，초성조이빈솔1 MHz，수출공솔1 W/cm2，지속복조90 s；초성미포조가입5μL함불탄기체지질체초성미포(약2×1011 L-1)，이동양초성조건작용90 s。 　　결과여결론：초성미포조혈관내피세포상청액적혈관내피생장인자화기질세포연생인자1표체수평균비대조조현저증고(P<0.05)。초성조혈관내피생장인자표체수평위여대조조무차이；이기질세포연생인자1위교대조조명현강저(P<0.01)。골수간충질간세포간예후CXCR4기인표체，초성미포조、초성조교대조조균명현증강(P<0.01)，단초성미포조화초성조무통계학차이(P>0.05)。설명초성(1 MHz，1 W/cm2)연합미포작용90 s능구촉진혈관내피세포분비세포인자(혈관내피생장인자화기질세포연생인자1)，동시촉진골수간충질간세포CXCR4기인표체，시기증강이식간세포귀소효응적궤제지일。
BACKGROUND:Animal studies have indicated ultrasound-mediated microbubbles can significantly enhance the effect of stem cel transplantation to treat ischemic diseases. But its mechanism is stil unknown. OBJECTIVE:To explore the mechanism of ultrasound-mediated microbubbles to significantly enhance the effect of stem cel transplantation in the treatment of ischemic diseases. METHODS:Bone marrow mesenchymal stem cel s and vascular endothelial cel s of rats were cultured in vitro, and then randomized to three groups:control group with no intervention, ultrasound group exposed to ultrasound at 1 MHz, 1 W/cm2 for 90 seconds, and ultrasound-mediated microbubble group treated with 5μL liposomes ultrasound microbubbles containing fluorocarbon gases (about 2×1011/L) and ultrasound exposure at 1 MHz, 1 W/cm2 for 90 seconds. RESULTS AND CONCLUSION:Compared to the control group, ultrasound-mediated microbubbles significantly increased expressions of vascular endothelial growth factor and stromal cel-derived factor 1 in the supernatant of vascular endothelial cel s (P<0.05);ultrasound had no effect on the expression of vascular endothelial growth factor, but decreased the level of stromal cel-derived factor 1 (P<0.01). Ultrasound-mediated microbubbles and the ultrasound alone could significantly enhance the CXCR4 gene expression in bone marrow mesenchymal stem cel s as compared with the control group (P<0.01), but there was no difference between the ultrasound-mediated microbubble group and the ultrasound group (P>0.05). These findings suggest that 1 W/cm2 ultrasound-mediated microbubbles can promote vascular endothelial growth factor and stromal cel-derived factor 1 secretion by vascular endothelia cel s, and meanwhile promote CXCR4 gene expression in bone marrow mesenchymal stem cel s. This may be the mechanism of the ultrasound-mediated microbubbles enhancing homing effect of transplanted stem cel s.