CAJ | 학술논문

背景：细胞因子在移植物抗宿主病的发生发展过程中起至关重要的作用，但目前缺乏其在重型β地中海贫血中的研究报道。 　　目的：探讨细胞因子与重型β地中海贫血患者造血干细胞移植后发生移植物抗宿主病之间的关系。 　　方法：采用液相芯片技术检测11例重型β地中海贫血移植物抗宿主病出现前，出现时，出现第4天，第7天，完全消失时白细胞介素6、白细胞介素8、白细胞介素12、肿瘤坏死因子α、巨噬细胞游走抑制因子的动态变化。 　　结果与结论：各时相白细胞介素6、白细胞介素12、肿瘤坏死因子α、巨噬细胞游走抑制因子水平差异有显著性意义，移植物抗宿主病出现时各细胞因子水平最高，移植物抗宿主病出现第7天次之。各时相白细胞介素8水平差异有显著性意义，移植物抗宿主病出现第4天最高。以上结果提示移植物抗宿主病发生前后白细胞介素6、白细胞介素8、白细胞介素12、肿瘤坏死因子α、巨噬细胞游走抑制因子动态水平变化在一定程度上反映移植后患者的免疫状态，可作为辅助诊断移植物抗宿主病的监测指标。
배경：세포인자재이식물항숙주병적발생발전과정중기지관중요적작용，단목전결핍기재중형β지중해빈혈중적연구보도。 　　목적：탐토세포인자여중형β지중해빈혈환자조혈간세포이식후발생이식물항숙주병지간적관계。 　　방법：채용액상심편기술검측11례중형β지중해빈혈이식물항숙주병출현전，출현시，출현제4천，제7천，완전소실시백세포개소6、백세포개소8、백세포개소12、종류배사인자α、거서세포유주억제인자적동태변화。 　　결과여결론：각시상백세포개소6、백세포개소12、종류배사인자α、거서세포유주억제인자수평차이유현저성의의，이식물항숙주병출현시각세포인자수평최고，이식물항숙주병출현제7천차지。각시상백세포개소8수평차이유현저성의의，이식물항숙주병출현제4천최고。이상결과제시이식물항숙주병발생전후백세포개소6、백세포개소8、백세포개소12、종류배사인자α、거서세포유주억제인자동태수평변화재일정정도상반영이식후환자적면역상태，가작위보조진단이식물항숙주병적감측지표。
BACKGROUND:Cytokines play an important role in the occurrence and development of graft-versus-host disease, but there is a current lack of reports on the association between cytokines and graft-versus-host disease after al ogeneic hematopoietic stem cel transplantation for treatment ofβ-thalassemia major. OBJECTIVE:To investigate the association between cytokines and graft-versus-host disease after al ogeneic hematopoietic stem cel transplantation forβ-thalassemia major. METHODS:We observed the dynamic variation of interleukin 6, interleukin 8, interleukin 12, tumor necrosis factor-αand macrophage migration inhibitory factor in 11 children withβ-thalassemia major before onset of graft-versus-host disease, when graft-versus-host disease occurred, at days 4 and 7 after onset of graft-versus-host disease, and when graft-versus-host disease disappeared. RESULTS AND CONCLUSION:There was a significant difference in serum levels of interleukin-6, interleukin-12, tumor necrosis factor-α, macrophage migration inhibitory factor in different time points, and the highest levels of different cytokines appeared when graft-versus-host disease occurred, fol owed by those at 7 days after graft-versus-host disease. There was a significant difference in serum levels of interleukin-8 in different time points, and the highest level appeared at 4 days after graft-versus-host disease. The dynamic expression of interleukin-6, interleukin-8, interleukin-12, tumor necrosis factor-α, macrophage migration inhibitory factor can estimate the immune function ofβ-thalassemia major patients who develops graft-versus-host disease after al ogeneic hematopoietic stem cel transplantation, and can be used as the immunobiology indicators for the early diagnosis of graft-versus-host disease.