CAJ | 학술논문

背景：鸡骨髓间充质干细胞是胚胎发育学、免疫学和肿瘤学研究重要的细胞模型，但如何大规模扩增并使其保持良好的未分化潜能是鸡骨髓间充质干细胞应用中亟待解决的难题。 　　目的：建立扩增鸡骨髓间充质干细胞的层粘连蛋白培养体系。 　　方法：将体外分离得到的鸡骨髓间充质干细胞分别接种在包被层粘连蛋白培养皿和传统二维培养皿上。经过体外扩增，比较两种培养体系下，鸡骨髓间充质干细胞的形态特征、表面标志物、扩增性能和成脂分化潜能。结果与结论：层粘连蛋白培养体系和常规培养体系中骨髓间充质干细胞的形态学特征和表面标志物表达没有显著差异，但层粘连蛋白培养体系获得的骨髓间充质干细胞增殖能力和分化潜能都明显优于传统培养体系。可见层粘连蛋白培养体系能快速地扩增出大量增殖能力强和未分化性能良好的鸡骨髓间充质干细胞，为鸡骨髓间充质干细胞的进一步深入研究和应用提供生物学基础。
배경：계골수간충질간세포시배태발육학、면역학화종류학연구중요적세포모형，단여하대규모확증병사기보지량호적미분화잠능시계골수간충질간세포응용중극대해결적난제。 　　목적：건립확증계골수간충질간세포적층점련단백배양체계。 　　방법：장체외분리득도적계골수간충질간세포분별접충재포피층점련단백배양명화전통이유배양명상。경과체외확증，비교량충배양체계하，계골수간충질간세포적형태특정、표면표지물、확증성능화성지분화잠능。결과여결론：층점련단백배양체계화상규배양체계중골수간충질간세포적형태학특정화표면표지물표체몰유현저차이，단층점련단백배양체계획득적골수간충질간세포증식능력화분화잠능도명현우우전통배양체계。가견층점련단백배양체계능쾌속지확증출대량증식능력강화미분화성능량호적계골수간충질간세포，위계골수간충질간세포적진일보심입연구화응용제공생물학기출。
BACKGROUND:Bone marrow mesenchymal stem cel s from chickens are important cel models for embryonic developmental biology, immunology and oncology research. However, it is difficult to keep bone marrow mesenchymal stem cel s with good undifferentiated potential in a large-scale expansion system. OBJECTIVE:To establish a culture system in vitro with laminin coating to expand bone marrow mesenchymal stem cel s from chickens. METHODS:Isolated bone marrow mesenchymal stem cel s from chickens were seeded in laminin-coated plates and traditional two-dimensional plates, respectively. After expansion in vitro, the morphological characteristics, expression of surface markers, expansion characteristics and adipogenic differentiation of bone marrow mesenchymal stem cel s in both conditions were analyzed and compared. RESULTS AND CONCLUSION:There were no statistical differences in the morphological characteristics and expression of surface markers of bone marrow mesenchymal stem cel s expanded by laminin-coated plates and traditional two-dimensional plates. But, the expansion characteristics and adipogenic differentiation of bone marrow mesenchymal stem cel s cultured in laminin-coated plates were better than those in traditional two-dimensional plates. Laminin culture system could quickly amplify out of a large number of chicken bone marrow mesenchymal stem cel s with better proliferation ability and undifferentiated performance. Al above results indicated that a more efficient expansion system with laminin coating is established.