CAJ | 학술논문

背景：课题组前期研究发现胚胎骨髓来源的间充质干细胞促进人Th17细胞增殖，但内在的调节机制尚需阐明。目的：探讨Tol 样受体3对胚胎骨髓来源间充质干细胞免疫调节Th17细胞的作用。 　　方法：应用免疫磁珠分离正常人 CD4+ T 细胞，单独或与胚胎来源骨髓间充质干细胞共培养4 d。实时定量PCR测定白细胞介素17、Tol 样受体3及MyD88相关基因的表达水平。 　　结果与结论：相对于CD4+T细胞单独培养组，间充质干细胞共培养组白细胞介素17 mRNA表达水平明显升高(3.59±0.11 vs.1.14±0.08，P <0.01)；进一步发现 Tol 样受体3 mRNA 亦相应升高(3.10±1.60 vs.0.94±0.01，P <0.05)。而且，相对于CD4+T细胞单独培养组，胚胎骨髓来源间充质干细胞共培养组促进了MyD88的表达(2.29±0.05 vs.1.85±0.31，P<0.01)。研究结果表明Tol 样受体3可能对胚胎骨髓来源间充质干细胞免疫调节Th17细胞发挥作用，为今后潜在的细胞治疗策略提供可能的实验依据。
배경：과제조전기연구발현배태골수래원적간충질간세포촉진인Th17세포증식，단내재적조절궤제상수천명。목적：탐토Tol 양수체3대배태골수래원간충질간세포면역조절Th17세포적작용。 　　방법：응용면역자주분리정상인 CD4+ T 세포，단독혹여배태래원골수간충질간세포공배양4 d。실시정량PCR측정백세포개소17、Tol 양수체3급MyD88상관기인적표체수평。 　　결과여결론：상대우CD4+T세포단독배양조，간충질간세포공배양조백세포개소17 mRNA표체수평명현승고(3.59±0.11 vs.1.14±0.08，P <0.01)；진일보발현 Tol 양수체3 mRNA 역상응승고(3.10±1.60 vs.0.94±0.01，P <0.05)。이차，상대우CD4+T세포단독배양조，배태골수래원간충질간세포공배양조촉진료MyD88적표체(2.29±0.05 vs.1.85±0.31，P<0.01)。연구결과표명Tol 양수체3가능대배태골수래원간충질간세포면역조절Th17세포발휘작용，위금후잠재적세포치료책략제공가능적실험의거。
BACKGROUND:Previous studies have found that embryonic bone marrow mesenchymal stem cel s can promote human Th17 cel proliferation, but the inherent regulatory mechanisms stil need to be elucidated. OBJECTIVE:To investigate the role of Tol-like receptor 3 in the immunoregulation of Th17 cel s by mesenchymal stem cel s. METHODS:Human CD4+T cel s from healthy donors were isolated by immunomagnetic bead method, and then cultured alone or co-cultured with embryonic bone marrow mesenchymal stem cel s for 4 days. The mRNA expression level of interleukin-17, Tol-like receptor 3 and MyD88 was detected by real-time PCR. RESULTS AND CONCLUSION:Compared with CD4+T cel cultured alone group, the mRNA level of interleukin-17 was significantly higher in the co-culture group (3.59±0.11 vs. 1.14± 0.08, P<0.01). Consistent with the expression of interleukin-17 mRNA, increased level of Tol-like receptor 3 mRNA was detected in the co-culture group compared with the CD4+T cel cultured alone group (3.10±1.60 vs. 0.94± 0.01, P<0.05). Furthermore, MyD88 in the co-culture group was significantly higher than that in CD4+T cel cultured alone group (2.29±0.05 vs. 1.85±0.31, P<0.01). Tol-like receptor 3 may be involved in the immunoregulation of Th17 cel s by embryonic bone marrow mesenchymal stem cel s, which provides experimental evidence for potential cel therapeutic strategy.