CAJ | 학술논문

目的：观察缺氧缺血性脑损伤（HIBD）大鼠脑组织海马区磷酸化tau（p-tau）蛋白及凋亡相关蛋白Bcl-2、Bax的表达，并分析其相关性。方法选择新生SD大鼠56只，随机分为正常对照组8只、假手术组8只和HIBD组40只（按处死时间点分为3、6、12、24、48 h 5个亚组，每组8只）。 HIBD组参照Rice法建模，并在不同时间点断头取脑。假手术组仅暴露左颈总动脉，正常对照组未做处理，均于手术结束后断头取脑。采用HE染色法观察各组脑组织海马区细胞形态结构改变，免疫组化染色法观察各组脑组织海马区p-tau、Bcl-2、Bax蛋白阳性细胞表达。结果正常对照组及假手术组脑组织细胞形态结构正常，基本无损伤性改变。 HIBD组在HIBD后3 h细胞无明显变化；HIBD后6 h出现细胞肿胀，结构排列紊乱；HIBD后24 h细胞肿胀明显，细胞核固缩、碎裂；HIBD后48 h细胞内出现凋亡小体，神经细胞数量锐减。 HIBD组各时间点脑组织海马区p-tau、Bcl-2、Bax蛋白阳性细胞数均较正常对照组和假手术组显著增多（P均＜0．05）；HIBD后12 h内，脑组织海马区p-tau蛋白阳性细胞数与Bcl-2、Bax蛋白阳性细胞数均呈正相关（ P均＜0．05）。结论 HIBD后脑组织海马区细胞启动了凋亡程序，p-tau蛋白参与了海马区细胞的凋亡过程，并且与Bcl-2、Bax蛋白的表达存在相关性。
목적：관찰결양결혈성뇌손상（HIBD）대서뇌조직해마구린산화tau（p-tau）단백급조망상관단백Bcl-2、Bax적표체，병분석기상관성。방법선택신생SD대서56지，수궤분위정상대조조8지、가수술조8지화HIBD조40지（안처사시간점분위3、6、12、24、48 h 5개아조，매조8지）。 HIBD조삼조Rice법건모，병재불동시간점단두취뇌。가수술조부폭로좌경총동맥，정상대조조미주처리，균우수술결속후단두취뇌。채용HE염색법관찰각조뇌조직해마구세포형태결구개변，면역조화염색법관찰각조뇌조직해마구p-tau、Bcl-2、Bax단백양성세포표체。결과정상대조조급가수술조뇌조직세포형태결구정상，기본무손상성개변。 HIBD조재HIBD후3 h세포무명현변화；HIBD후6 h출현세포종창，결구배렬문란；HIBD후24 h세포종창명현，세포핵고축、쇄렬；HIBD후48 h세포내출현조망소체，신경세포수량예감。 HIBD조각시간점뇌조직해마구p-tau、Bcl-2、Bax단백양성세포수균교정상대조조화가수술조현저증다（P균＜0．05）；HIBD후12 h내，뇌조직해마구p-tau단백양성세포수여Bcl-2、Bax단백양성세포수균정정상관（ P균＜0．05）。결론 HIBD후뇌조직해마구세포계동료조망정서，p-tau단백삼여료해마구세포적조망과정，병차여Bcl-2、Bax단백적표체존재상관성。
Objective To observe the expression of phosphorylated tau ( p-tau) protein, and apoptosis related pro-teins Bcl-2 and Bax, and explore their correlations .Methods Seven-day-old newborn clean Sprague Dawley ( SD) rats, were randomly divided into normal control group (8 cases), sham operation (8 cases), and HIBD group (which was divid-ed into by death time points 3, 6, 12, 24, 48 h subsection 8 cases in each group).HIBD animal model was established by Rice method, and took the brain at different time points .In sham group, rats were just exposed the left carotid artery , and in the control group rats did not dispose .Rats were all decapitated brain immediately at the end of surgery .Hematoxylin-e-osin ( HE) staining was used to view morphological changes in the hippocampus , and immunohistochemical staining was used to test the expressions of p-tau, Bcl-2 and Bax protein.Results Brain tissues in the normal control group and sham operation group was normal .In HIBD group, the cell has not significant change 3 h after HIBD;Cell swelling and structur-al disorder 6 h after HIBD; Cell swelling significantly , nuclear condensation , fragmentation 24 h after HIBD;48 h after HIBD, apoptotic bodies occured , the number of nerve cells was dropped .In HIBD group, Bcl-2 and Bax positive cells in HIBD group at each time point were more than those in the normal control group and the sham group (all P<0.05);In the hippocampus of HIBD group , p-tau positive cells was positively correlated with Bcl-2 and Bax positive cells during 12 h af-ter HIBD(P<0.05).Conclusion The hippocampus neurolcytes actiwated apotosis mechanism afer HIBD early period . P-tau protein was involved in neuronal apoptosis after HIBD , and there is connection among p-tau, Bcl-2 and Bax protein.