浙江海洋学院学报（自然科学版）
浙江海洋學院學報（自然科學版）
절강해양학원학보（자연과학판）
JOURNAL OF ZHEJIANG OCEAN UNIVERSITY（NATURAL SCIENCE）
2014年
4期
298-303
,共6页
李斌%马元庆%邢红艳%靳洋%刘爱英%白艳艳%孙珊%张娟%何鑫
李斌%馬元慶%邢紅豔%靳洋%劉愛英%白豔豔%孫珊%張娟%何鑫
리빈%마원경%형홍염%근양%류애영%백염염%손산%장연%하흠
刺参%Zn2+%Mn7+%急性毒性%富集作用
刺參%Zn2+%Mn7+%急性毒性%富集作用
자삼%Zn2+%Mn7+%급성독성%부집작용
Apostichopus japonicas%Zn2+%Mn7+%acute toxicity%enrichment
在静水条件下，研究了Zn2+、Mn7+两种重金属离子对刺参幼参行为及其存活状况的影响，并分析了其在幼参体内的富集状况。结果表明：Zn2+、Mn7+单独处理时，幼参在Zn2+浓度低于0.4 mg/L或Mn7+浓度低于0.5 mg/L条件下暴露96 h后死亡率均为0.0%，表明在此对应浓度范围内两种金属离子均无明显的急性毒性作用；随浓度和暴露时间增加，幼参死亡率明显升高，其附壁率呈降低趋势。Zn2+对幼参的24、48、72 h的半致死浓度LC50分别为49.70、1.35、0.82 mg/L，安全浓度（SC）为0.14 mg/L；Mn7+对幼参的48、72、96 h的半致死浓度LC50分别为31.61、4.24、3.13 mg/L，SC为3.16 mg/L。因此，Zn2+对幼参的急性毒性作用强于Mn7+。随着水体中Zn2+与Mn7+浓度的增加，幼参体内重金属元素含量和累积速率总体呈升高趋势，而富集系数呈降低趋势，Zn2+的富集作用强于Mn7+。
在靜水條件下，研究瞭Zn2+、Mn7+兩種重金屬離子對刺參幼參行為及其存活狀況的影響，併分析瞭其在幼參體內的富集狀況。結果錶明：Zn2+、Mn7+單獨處理時，幼參在Zn2+濃度低于0.4 mg/L或Mn7+濃度低于0.5 mg/L條件下暴露96 h後死亡率均為0.0%，錶明在此對應濃度範圍內兩種金屬離子均無明顯的急性毒性作用；隨濃度和暴露時間增加，幼參死亡率明顯升高，其附壁率呈降低趨勢。Zn2+對幼參的24、48、72 h的半緻死濃度LC50分彆為49.70、1.35、0.82 mg/L，安全濃度（SC）為0.14 mg/L；Mn7+對幼參的48、72、96 h的半緻死濃度LC50分彆為31.61、4.24、3.13 mg/L，SC為3.16 mg/L。因此，Zn2+對幼參的急性毒性作用彊于Mn7+。隨著水體中Zn2+與Mn7+濃度的增加，幼參體內重金屬元素含量和纍積速率總體呈升高趨勢，而富集繫數呈降低趨勢，Zn2+的富集作用彊于Mn7+。
재정수조건하，연구료Zn2+、Mn7+량충중금속리자대자삼유삼행위급기존활상황적영향，병분석료기재유삼체내적부집상황。결과표명：Zn2+、Mn7+단독처리시，유삼재Zn2+농도저우0.4 mg/L혹Mn7+농도저우0.5 mg/L조건하폭로96 h후사망솔균위0.0%，표명재차대응농도범위내량충금속리자균무명현적급성독성작용；수농도화폭로시간증가，유삼사망솔명현승고，기부벽솔정강저추세。Zn2+대유삼적24、48、72 h적반치사농도LC50분별위49.70、1.35、0.82 mg/L，안전농도（SC）위0.14 mg/L；Mn7+대유삼적48、72、96 h적반치사농도LC50분별위31.61、4.24、3.13 mg/L，SC위3.16 mg/L。인차，Zn2+대유삼적급성독성작용강우Mn7+。수착수체중Zn2+여Mn7+농도적증가，유삼체내중금속원소함량화루적속솔총체정승고추세，이부집계수정강저추세，Zn2+적부집작용강우Mn7+。
Using static bioassay test, this paper studied the acute toxicity of Zn 2+ and Mn7+ on behavior and survival status of the juvenile sea cucumber Apostichopus japonicas, furthermore, the accumulation in A. japonicas was also analyzed. The results showed that 96 h separating exposure to Zn2+ or Mn7+, the mortality rates of A. japonicas in Zn2+under 0.4 mg/L and Mn7+under 0.5 mg/L were 0.0%, and no obvious acute toxici-ty was found within the above experimental concentration; The mortality rate increased with the increase of concentration and exposure time, but the adhesion rate decreased. The median lethal concentrations ( LC50) of Zn2+in 24 h, 48 h and 72 h were 49.70, 1.35 and 0.82 mg/L respectively, and its safety concentration (SC) was 0.14 mg/L; The median lethal concentrations (LC50) of Mn7+in 48 h, 72 h and 96 h were 31.61, 4.24 and 3.13 mg/L, and the SC was 3.16 mg/L. Therefore, it was concluded that Zn2+had a stronger acute toxicity than Mn7+on A. japonicas. With increasing concentrations of Zn2+ and Mn7+, the contents of hmetals and accumulation rates gradually increased, but the change of enrichment coefficient wsa contrary. It was indicated that Zn2+showed the stronger accumulating process than Mn7+on the juvenile sea cucumber.
