CAJ | 학술논문

目的：通过观察环维黄杨星D( CVB_D)对心力衰竭(心衰)大鼠心脏功能和心肌形态、结构的影响,探讨CVB_D对心衰的治疗作用。方法结扎SD大鼠冠脉左前降支复制大鼠心衰模型,随机分为5组,另加假手术组。应用超声心动仪检测心功能指标：左室后壁舒张期厚度( LVPWd )、左室后壁收缩期厚度( LVPWs )、左室收缩末期内径( LVIDs)、左室舒张末期内径( LVIDd)、射血分数( EF)、心率( HR)、心输出量( CO)、左室短轴缩短率( LVSF)、每搏输出量( SV)；制作心肌组织切片,苏木精_伊红( HE)染色观察心肌组织学改变,透射电镜观察心肌超微结构的变化。结果与模型对照组比较,CVB_D中、大剂量组EF、 LVSF明显增加(P<0.05或P<0.01),CVB_D小、中剂量组可见SV增加(P<0.05),CVB_D小、中、大剂量组显示CO增加(P<0.05),CVB_D大剂量组显示LVIDs减少,LVPWs增加(P<0.05)。光镜下CVB_D各剂量组使受损心肌组织出现不同程度的变化：心肌纤维排列逐渐整齐,横纹逐渐明显,轮廓逐渐清晰。透射电镜下心肌超微结构发生变化：CVB_D使受损心肌纤维断裂减少,线粒体数量增多、肿胀变性减轻。结论 CVB_D对心衰大鼠心脏收缩、泵血功能有明显的改善作用,对心肌肌纤维、线粒体的损伤有缓解作用,推测CVB_D对心衰存在一定的治疗作用。
목적：통과관찰배유황양성D( CVB_D)대심력쇠갈(심쇠)대서심장공능화심기형태、결구적영향,탐토CVB_D대심쇠적치료작용。방법결찰SD대서관맥좌전강지복제대서심쇠모형,수궤분위5조,령가가수술조。응용초성심동의검측심공능지표：좌실후벽서장기후도( LVPWd )、좌실후벽수축기후도( LVPWs )、좌실수축말기내경( LVIDs)、좌실서장말기내경( LVIDd)、사혈분수( EF)、심솔( HR)、심수출량( CO)、좌실단축축단솔( LVSF)、매박수출량( SV)；제작심기조직절편,소목정_이홍( HE)염색관찰심기조직학개변,투사전경관찰심기초미결구적변화。결과여모형대조조비교,CVB_D중、대제량조EF、 LVSF명현증가(P<0.05혹P<0.01),CVB_D소、중제량조가견SV증가(P<0.05),CVB_D소、중、대제량조현시CO증가(P<0.05),CVB_D대제량조현시LVIDs감소,LVPWs증가(P<0.05)。광경하CVB_D각제량조사수손심기조직출현불동정도적변화：심기섬유배렬축점정제,횡문축점명현,륜곽축점청석。투사전경하심기초미결구발생변화：CVB_D사수손심기섬유단렬감소,선립체수량증다、종창변성감경。결론 CVB_D대심쇠대서심장수축、빙혈공능유명현적개선작용,대심기기섬유、선립체적손상유완해작용,추측CVB_D대심쇠존재일정적치료작용。
Objective To exPlore the theraPeutic effect of cycloVirobuxine_D ( CVB_D) on heart failure by obserVing the influences of CVB_D on cardiac function,myocardial morPhology and structure in rats with heart failure. Methods The rat model of heart failure was established by ligating left anterior descending coronary artery,and then the rats were randomly diVided into 5 grouPs,Plus the sham grouP. EchocardiograPhy was used to assess cardiac function,including EF,LVSF,SV,HR,CO and so on. Myocardial tissue section was obtained. Change in the myocardial tissue was obserVed by HE staining. Myocardial ultra_structure was obserVed by using transmission electron microscoPe. Results As comPared with the model grouP,EF and LVSF were significantly increased in medium and high dose CVB_D grouPs (P<0. 05 or P<0. 01),SV was increased in low and medium dose CVB_D grouPs (P<0. 05),CO was increased in low,medium and high dose CVB_D grouPs (P<0. 05),LVIDs was decreased and LVPWs was increased in high dose CVB_D grouP (P<0. 05). Under light microscoPe,changes of myocardial structures were obserVed in different CVB_D dose grouPs: myocardial fiber gradually became tidy, striPes became clear, and contour became clear. The changes of myocardial ultra_structure were obserVed under transmission electron microscoPe: the myocardial fiber breakage was reduced and the number of mitochondria was increased,swelling degeneration was alleViated. Conclusion CVB_D significantly imProVed cardiac contraction and blood PumPing function of rats with heart failure, exerted mitigatiVe effect on damage of cardiac muscle fiber and mitochondria. It suggests that CVB_D may be effectiVe in the treatment of heart failure.