临床肝胆病杂志
臨床肝膽病雜誌
림상간담병잡지
CHINESE JOURNAL OF CLINICAL HEPATOLOGY
2014年
12期
1303-1306
,共4页
周宁%张月荣%魏世芳%吴丽阳%李象霖
週寧%張月榮%魏世芳%吳麗暘%李象霖
주저%장월영%위세방%오려양%리상림
肝炎,乙型,慢性%雌激素受体α%多态性,单核苷酸%甘肃%汉族%回族
肝炎,乙型,慢性%雌激素受體α%多態性,單覈苷痠%甘肅%漢族%迴族
간염,을형,만성%자격소수체α%다태성,단핵감산%감숙%한족%회족
hepatitis B,chronic%estrogen receptor alpha%polymorphism,single nucleotide%GANSU%HAN NATIONALITY%HUI NA-TIONALITY
目的：分析甘肃地区汉族和回族慢性乙型肝炎（CHB）患者雌激素受体（ER）α－29位基因多态性的分布情况。方法选取2012年8月至2013年11月在兰州市第一人民医院就诊的甘肃地区回族及汉族CHB患者各84例。采用聚合酶链反应－限制性片段长度多态性法检测ERα－29位多态性。用基因计数法计算检验人群的等位基因频率，进行Hardy－Weinberg遗传平衡定律检验。应用非条件Logistic回归分析将混杂因素进行调整。基因型及等位基因频率比较采用卡方检验。结果甘肃省汉族、回族CHB 人群的ERα－29位点基因型频率：TT为52．4％、60．7％；TC为40．5％、34．5％；CC为7．1％、4．8％。等位基因频率：T为72．6％、78.0％；C为27．4％、22．0％。ERα－29位基因型频率在甘肃汉族、回族CHB患者间的差异无统计学意义（P＞0．05）。结论 ERα－29位基因多态性在甘肃省汉族、回族CHB人群中无民族差异。
目的：分析甘肅地區漢族和迴族慢性乙型肝炎（CHB）患者雌激素受體（ER）α－29位基因多態性的分佈情況。方法選取2012年8月至2013年11月在蘭州市第一人民醫院就診的甘肅地區迴族及漢族CHB患者各84例。採用聚閤酶鏈反應－限製性片段長度多態性法檢測ERα－29位多態性。用基因計數法計算檢驗人群的等位基因頻率，進行Hardy－Weinberg遺傳平衡定律檢驗。應用非條件Logistic迴歸分析將混雜因素進行調整。基因型及等位基因頻率比較採用卡方檢驗。結果甘肅省漢族、迴族CHB 人群的ERα－29位點基因型頻率：TT為52．4％、60．7％；TC為40．5％、34．5％；CC為7．1％、4．8％。等位基因頻率：T為72．6％、78.0％；C為27．4％、22．0％。ERα－29位基因型頻率在甘肅漢族、迴族CHB患者間的差異無統計學意義（P＞0．05）。結論 ERα－29位基因多態性在甘肅省漢族、迴族CHB人群中無民族差異。
목적：분석감숙지구한족화회족만성을형간염（CHB）환자자격소수체（ER）α－29위기인다태성적분포정황。방법선취2012년8월지2013년11월재란주시제일인민의원취진적감숙지구회족급한족CHB환자각84례。채용취합매련반응－한제성편단장도다태성법검측ERα－29위다태성。용기인계수법계산검험인군적등위기인빈솔，진행Hardy－Weinberg유전평형정률검험。응용비조건Logistic회귀분석장혼잡인소진행조정。기인형급등위기인빈솔비교채용잡방검험。결과감숙성한족、회족CHB 인군적ERα－29위점기인형빈솔：TT위52．4％、60．7％；TC위40．5％、34．5％；CC위7．1％、4．8％。등위기인빈솔：T위72．6％、78.0％；C위27．4％、22．0％。ERα－29위기인형빈솔재감숙한족、회족CHB환자간적차이무통계학의의（P＞0．05）。결론 ERα－29위기인다태성재감숙성한족、회족CHB인군중무민족차이。
Objective To investigate the genetic polymorphisms in the estrogen receptor (ER)alpha gene at position 29 (ERα-29)in pa-tients with chronic hepatitis B (CHB)from Han nationality and Hui minority populations in Gansu Province,China.Methods Polymorphisms of ERα-29 were analyzed in 84 Han and 84 Hui CHB patients who were admitted to our hospital from August 2012 to November 2013 by poly-merase chain reaction-restriction fragment length polymorphism analysis.Allele frequencies were determined by gene counting and tested for expectations of Hardy-Weinberg equilibrium.Unconditional logistic regression analysis was applied to adjust the confounding factors.Compar-ison of the genotype and allele frequencies was performed by chi-square test.Results The genotype frequencies of ERα-29 in Han versus Hui CHB patients from Gansu Province were as follows:TT,52.4%vs 60.7%;TC,40.5%vs 34.5%;CC,7.1%vs 4.8%.The allele fre-quencies were 72.6%vs 78.0%for the T allele and 27.4%vs 22.0%for the C allele.No significant differences in the allele and genotype frequencies of ERα-29 were found between Han and Hui CHB patients in Gansu Province (P>0.05 ).Conclusion The genetic polymor-phisms in ERα-29 are not significantly different between Han and Hui CHB patients in Gansu Province,China.
