CAJ | 학술논문

目的：探讨老龄SHR大鼠胸主动脉过氧化物酶体增殖物激活受体γ（ PPARγ）表达变化情况及罗格列酮（Ros）干预作用。方法：64周龄Wistar‐Kyoto（WKY）大鼠和自发性高血压大鼠（ SHR）分为WKY ，SHR及SHR＋Ros组，每组9只。SHR＋Ros组大鼠给予Ros（5mg／kg／d）灌胃，连续56d；WKY和SHR组大鼠分别灌服等体积的生理盐水。干预结束后采用tail cuff法测量各组大鼠尾动脉收缩压，采用HE染色观察主动脉组织病理学改变情况，采用免疫组化染色及图像分析检测胸主动脉PPARγ蛋白表达情况，采用real time‐PCR检测主动脉PPARγ mRNA表达情况。结果：与WKY大鼠相比，SHR大鼠血压升高（P＜0．05），HE染色可见胸主动脉管壁增厚，内弹力板变直，间质增厚，细胞核着色较淡，免疫组化染色及real time‐PCR结果表明SHR大鼠胸主动脉PPARγ蛋白及mRNA表达水平均下降（均 P＜0．05），与SHR组相比，SHR＋Ros组大鼠血压降低（P＜0．05），胸主动脉组织病理学改变情况缓解，管壁变薄，内弹力板趋于迂曲，间质变薄，细胞核着色变深，PPARγ蛋白及mRNA表达水平均增加（P＜0．05）。结论：老龄SHR大鼠胸主动脉存在PPARγ表达下降，PPARγ配体罗格列酮可以逆转这种现象。
목적：탐토노령SHR대서흉주동맥과양화물매체증식물격활수체γ（ PPARγ）표체변화정황급라격렬동（Ros）간예작용。방법：64주령Wistar‐Kyoto（WKY）대서화자발성고혈압대서（ SHR）분위WKY ，SHR급SHR＋Ros조，매조9지。SHR＋Ros조대서급여Ros（5mg／kg／d）관위，련속56d；WKY화SHR조대서분별관복등체적적생리염수。간예결속후채용tail cuff법측량각조대서미동맥수축압，채용HE염색관찰주동맥조직병이학개변정황，채용면역조화염색급도상분석검측흉주동맥PPARγ단백표체정황，채용real time‐PCR검측주동맥PPARγ mRNA표체정황。결과：여WKY대서상비，SHR대서혈압승고（P＜0．05），HE염색가견흉주동맥관벽증후，내탄력판변직，간질증후，세포핵착색교담，면역조화염색급real time‐PCR결과표명SHR대서흉주동맥PPARγ단백급mRNA표체수평균하강（균 P＜0．05），여SHR조상비，SHR＋Ros조대서혈압강저（P＜0．05），흉주동맥조직병이학개변정황완해，관벽변박，내탄력판추우우곡，간질변박，세포핵착색변심，PPARγ단백급mRNA표체수평균증가（P＜0．05）。결론：노령SHR대서흉주동맥존재PPARγ표체하강，PPARγ배체라격렬동가이역전저충현상。
Objectives:To investigate the expression of peroxisome proliferators‐activated receptor γ(PPARγ) in the thoracic aorta of spontaneously hypertensive rats (SHR) with age and the inhibitory effect of rosigl‐itazone (Ros) on the expression of PPARγ.Methods :64‐week old wistar‐Kyoto rats (WKY) and SHR were ran‐domly and respectively divided into WKY ,SHR and SHR+ Ros group ,with 9 rats in each group .The rats in SHR+ Ros group were treated with Ros (5 mg/kg ,intragastrically) for 56 days ,whereas normal saline was applied in WKY and SHR groups .Systolic blood pressure (SBP) of rats was measured by tail cuff method .Histopathological damage of thoracic aorta was analyzed by Hematoxylin‐eosin (HE) staining .The levels of PPARγprotein and mR‐NA of thoracic aorta were detected by immunohistochemistry staining and real time‐PCR respectively .Results :Com‐pared with the rats in WKY group ,the SBP of rats in SHR group was higher (P<0 .05) ,and the levels of PPARγprotein and mRNA in thoracic aorta of rats in SHR group were lower respectively (P<0 .05) .In addition ,HE stai‐ning revealed some remarkable histopathological abnormalities in thoracic aorta of rats in SHR group .The thickness of thoracic aorta was increased ,and the elastic fiber was straight ,and the interstitium between elastic fibers become thick ,and the nucleuses were stained by hematoxylin slightly .Compared with the rats in SHR group ,the SBP of rats in SHR+Ros group was lower (P<0 .05) ,and the histopathological abnormalities in thoracic aorta were atten‐uated .The thickness of thoracic aorta was decreased ,and the elastic fiber was twisted ,and the interstitium between elastic fibers become thin ,and nucleus could be stained by hematoxylin deeply .Furthermore ,the levels of PPARγprotein and mRNA in thoracic aorta of rats in SHR group were higher than those in SHR group respectively (P<0 . 05) .Conclusion:The expression of PPARγin the thoracic aorta of SHR with age is decreased while thoracic aorta is aging ,and rosiglitazone can play an inhibitory effect .