CAJ | 학술논문

耐辐射奇异球菌（Deinococcus wulumuqiensis ）R12在环境胁迫下具有合成海藻糖的能力。采用 PCR 方法从耐辐射奇异球菌 R12基因组中分离得到分子量约1700 bp 的海藻糖合成酶基因，为验证其功能而在大肠杆菌中进行了表达，经 IPTG 诱导表达出约66 kDa 的目的蛋白。经酶活检测发现，目的蛋白能够催化麦芽糖一步合成海藻糖。以30％麦芽糖为底物反应1 h，进行海藻糖转化实验，结果表明，重组海藻糖合成酶的最适反应温度为35℃、最适反应 pH值为8.0，转化率达67％，具有较高的应用价值。
내복사기이구균（Deinococcus wulumuqiensis ）R12재배경협박하구유합성해조당적능력。채용 PCR 방법종내복사기이구균 R12기인조중분리득도분자량약1700 bp 적해조당합성매기인，위험증기공능이재대장간균중진행료표체，경 IPTG 유도표체출약66 kDa 적목적단백。경매활검측발현，목적단백능구최화맥아당일보합성해조당。이30％맥아당위저물반응1 h，진행해조당전화실험，결과표명，중조해조당합성매적최괄반응온도위35℃、최괄반응 pH치위8.0，전화솔체67％，구유교고적응용개치。
Deinococcus wulumuqiensis R12 has the ability to produce trehalose under the extreme condi-tions.1 700 bp DNA fragment encoding trehalose synthase was cloned from D.wulumuqiensis R12 by PCR method.Then it was expressed in E.coli to verify its function.By adding IPTG to induce the recombinant plas-mid,a novel target protein band of 66 kDa was detected by SDS-PAGE analysis.After determination of the en-zymatic activity,it was found that this trehalose synthase could catalyze the reversible interconversion of malt-ose and trehalose.When using 30% maltose as substrate,reacting for 1 h,it was found that the optimum reac-tion temperature and pH value for recombinant trehalose synthase was 35 ℃,8.0,respectively,the conversion rate reached 67%.