中国中西医结合外科杂志
中國中西醫結閤外科雜誌
중국중서의결합외과잡지
CHINESE JOURNAL OF SURGERY OF INTEGRATED TRADITIONAL AND WESTERN MEDICINE
2014年
6期
607-611
,共5页
刘礼青%王德华%王彬%李霞%田楠楠
劉禮青%王德華%王彬%李霞%田楠楠
류례청%왕덕화%왕빈%리하%전남남
绿原酸%Th1/Th2亚群%深静脉血栓形成%细胞因子
綠原痠%Th1/Th2亞群%深靜脈血栓形成%細胞因子
록원산%Th1/Th2아군%심정맥혈전형성%세포인자
Chlorogenic acid%Th1/Th2 subsets%deep vein thrombosis%cytokines
目的：探讨Th1/Th2亚群失衡在大鼠深静脉血栓形成（DVT）中的作用及绿原酸的作用机制。方法：40只DVT大鼠随机分为DVT模型组、绿原酸小剂量组、绿原酸中剂量组、绿原酸大剂量组，每组各10只，分离大鼠脾脏单核细胞，流式细胞术检测各组大鼠Th1亚群（CD4+TNF-α+）、Th2亚群（CD4+IL-4+）比例，ELISA法检测大鼠外周血IL-2、TNF-α、IL-4、IL-10水平，并分析Th1/Th2比例与血清炎症标记物相关性。结果：与正常组及假手术组比较，DVT模型组大鼠脾脏Th1亚群比例升高（22.71±0.87，%），Th2亚群比例降低（3.06±0.15，%），Th1/Th2升高（7.20±0.45），IL-2、TNF-α表达升高（72.10±7.48，173.50±17.02, pg/mL），IL-4、IL-10表达降低（347.46±11.24、28.55±1.78,pg/mL）；与模型组比较，绿原酸大剂量组大鼠Th1亚群比例降低（14.38±0.41,%），Th2亚群比例升高（4.91±0.14,%），Th1/Th2降低（3.02±0.26），IL-2、TNF-α表达降低（50.22±4.06、110.22±10.32, pg/mL），IL-4、IL-10表达升高（418.72±15.32、9.32±2.83,pg/mL），绿原酸大剂量组疗效优于中、小剂量组（P＜0.05）；Th1/Th2比例与IL-2、TNF-α呈正相关，与IL-4、IL-10呈负相关。结论：大鼠DVT发病过程中存在Th1/Th2亚群失衡，绿原酸诱导T细胞向Th2亚群偏移，逆转Th1/Th2亚群失衡状态，进而减轻炎症反应，抑制血栓形成。
目的：探討Th1/Th2亞群失衡在大鼠深靜脈血栓形成（DVT）中的作用及綠原痠的作用機製。方法：40隻DVT大鼠隨機分為DVT模型組、綠原痠小劑量組、綠原痠中劑量組、綠原痠大劑量組，每組各10隻，分離大鼠脾髒單覈細胞，流式細胞術檢測各組大鼠Th1亞群（CD4+TNF-α+）、Th2亞群（CD4+IL-4+）比例，ELISA法檢測大鼠外週血IL-2、TNF-α、IL-4、IL-10水平，併分析Th1/Th2比例與血清炎癥標記物相關性。結果：與正常組及假手術組比較，DVT模型組大鼠脾髒Th1亞群比例升高（22.71±0.87，%），Th2亞群比例降低（3.06±0.15，%），Th1/Th2升高（7.20±0.45），IL-2、TNF-α錶達升高（72.10±7.48，173.50±17.02, pg/mL），IL-4、IL-10錶達降低（347.46±11.24、28.55±1.78,pg/mL）；與模型組比較，綠原痠大劑量組大鼠Th1亞群比例降低（14.38±0.41,%），Th2亞群比例升高（4.91±0.14,%），Th1/Th2降低（3.02±0.26），IL-2、TNF-α錶達降低（50.22±4.06、110.22±10.32, pg/mL），IL-4、IL-10錶達升高（418.72±15.32、9.32±2.83,pg/mL），綠原痠大劑量組療效優于中、小劑量組（P＜0.05）；Th1/Th2比例與IL-2、TNF-α呈正相關，與IL-4、IL-10呈負相關。結論：大鼠DVT髮病過程中存在Th1/Th2亞群失衡，綠原痠誘導T細胞嚮Th2亞群偏移，逆轉Th1/Th2亞群失衡狀態，進而減輕炎癥反應，抑製血栓形成。
목적：탐토Th1/Th2아군실형재대서심정맥혈전형성（DVT）중적작용급록원산적작용궤제。방법：40지DVT대서수궤분위DVT모형조、록원산소제량조、록원산중제량조、록원산대제량조，매조각10지，분리대서비장단핵세포，류식세포술검측각조대서Th1아군（CD4+TNF-α+）、Th2아군（CD4+IL-4+）비례，ELISA법검측대서외주혈IL-2、TNF-α、IL-4、IL-10수평，병분석Th1/Th2비례여혈청염증표기물상관성。결과：여정상조급가수술조비교，DVT모형조대서비장Th1아군비례승고（22.71±0.87，%），Th2아군비례강저（3.06±0.15，%），Th1/Th2승고（7.20±0.45），IL-2、TNF-α표체승고（72.10±7.48，173.50±17.02, pg/mL），IL-4、IL-10표체강저（347.46±11.24、28.55±1.78,pg/mL）；여모형조비교，록원산대제량조대서Th1아군비례강저（14.38±0.41,%），Th2아군비례승고（4.91±0.14,%），Th1/Th2강저（3.02±0.26），IL-2、TNF-α표체강저（50.22±4.06、110.22±10.32, pg/mL），IL-4、IL-10표체승고（418.72±15.32、9.32±2.83,pg/mL），록원산대제량조료효우우중、소제량조（P＜0.05）；Th1/Th2비례여IL-2、TNF-α정정상관，여IL-4、IL-10정부상관。결론：대서DVT발병과정중존재Th1/Th2아군실형，록원산유도T세포향Th2아군편이，역전Th1/Th2아군실형상태，진이감경염증반응，억제혈전형성。
Objective To evaluate the role of Th1/Th2 shift in deep venous thrombosis (DVT) and eluci?date the mechanism of chlorogenic acid in treating DVT in rat models. Method Forty DVT rats were divided randomly into DVT group and chlorogenic acid high dose, middle dose and low dose groups. Additionally, 10 rats with sham operation were taken as sham operation group and 10 normal rats were taken as normal group re?spectively. Rats of chlorogenic acid group were treated with chlorogenic acid for 7 days, while rats of other groups were treated with physiological saline for the same duration. Then, the proportions of Th1 (CD4+TNF-α+) subset and Th2 (CD4+IL-4+) subset were detected by flowcytometry. Moreover, the protein expressions of IL-2, TNF-α, IL-4 and IL-10 in rat serum were analyzed by ELISA assay, and the proportion of Th1/ Th2 correlated with serum levels of inflammatory markers was analyzed. Results Compared with normal and sham operation groups, the proportion of Th1 subset was increased while that of Th2 subset was decreased significantly in DVT group(22.71 ± 0.87%,3.06 ± 0.15%). In addition, pro?tein expression of IL-2 and TNF-α was enhanced (72.10 ± 7.48 173.50 ± 17.02, pg/mL)while that of IL-4 and IL-10 was inhibited markedly in DVT rats (347.46 ± 11.24 28.55 ± 1.78,pg/mL). After chlorogen?ic acid administration, the proportion of Th1 subset was down-regulated while that of Th2 subset was up-regulated significantly in DVT rats(14.38 ± 0.4 14.91 ± 0.14, %). Moreover, the protein expression of IL-2 and TNF-α decreased while that of IL-4 and IL-10 increased obviously after chlorogenic acid administra?tion, Th1/ Th2 proportional positively correlated with IL-2 and TNF-α, and negatively correlated with IL-4, IL-10(50.22 ± 4.06 110.22 ± 10.32, 418.72 ± 15.32 9.32 ± 2.83,pg/mL). Conclusion Th1/Th2 shift is involved in DVT. Chlorogenic acid can down-regulate the Th1 subsets and up-regulate the Th2 subsets and exert the cu?rative effect on DVT mice.
