郑州轻工业学院学报(自然科学版)
鄭州輕工業學院學報(自然科學版)
정주경공업학원학보(자연과학판)
JOURNAL OF ZHENGZHOU INSTITUTE OF LIGHT INDUSTRY(NATURAL SCIENCE)
2014年
6期
33-38
,共6页
陈冠男%刘金香%曹宇%王红星
陳冠男%劉金香%曹宇%王紅星
진관남%류금향%조우%왕홍성
菠菜%气孔开度%盐胁迫%水杨酸
菠菜%氣孔開度%鹽脅迫%水楊痠
파채%기공개도%염협박%수양산
Spinacia oleracea L%stomatal aperture%salt stress%salicylic acid
以日本大叶菠菜为材料,采用土培法,研究了不同浓度水杨酸(SA)对盐胁迫下菠菜叶表皮气孔开度的调节作用.利用扫描电镜、普通光学显微镜及蒽酮法对菠菜叶表皮气孔形态、气孔开度及可溶性糖含量进行测试和表征.实验结果表明:随着SA浓度的增大,叶表皮气孔形态由细长线形逐渐变成椭圆形;叶表皮气孔开度逐渐增大,当SA浓度为200 mg/L时,气孔开度最大;叶表皮可溶性糖含量呈先增加后降低的变化趋势,当SA浓度为150 mg/L时,可溶性糖含量达到最大,比盐胁迫下的含量增加了28.9%.因此,SA的加入可以调节菠菜叶表皮的气孔形态和气孔开度,提高其光合效率,改善其渗透调节作用,从而增强菠菜的抗盐性.
以日本大葉菠菜為材料,採用土培法,研究瞭不同濃度水楊痠(SA)對鹽脅迫下菠菜葉錶皮氣孔開度的調節作用.利用掃描電鏡、普通光學顯微鏡及蒽酮法對菠菜葉錶皮氣孔形態、氣孔開度及可溶性糖含量進行測試和錶徵.實驗結果錶明:隨著SA濃度的增大,葉錶皮氣孔形態由細長線形逐漸變成橢圓形;葉錶皮氣孔開度逐漸增大,噹SA濃度為200 mg/L時,氣孔開度最大;葉錶皮可溶性糖含量呈先增加後降低的變化趨勢,噹SA濃度為150 mg/L時,可溶性糖含量達到最大,比鹽脅迫下的含量增加瞭28.9%.因此,SA的加入可以調節菠菜葉錶皮的氣孔形態和氣孔開度,提高其光閤效率,改善其滲透調節作用,從而增彊菠菜的抗鹽性.
이일본대협파채위재료,채용토배법,연구료불동농도수양산(SA)대염협박하파채협표피기공개도적조절작용.이용소묘전경、보통광학현미경급은동법대파채협표피기공형태、기공개도급가용성당함량진행측시화표정.실험결과표명:수착SA농도적증대,협표피기공형태유세장선형축점변성타원형;협표피기공개도축점증대,당SA농도위200 mg/L시,기공개도최대;협표피가용성당함량정선증가후강저적변화추세,당SA농도위150 mg/L시,가용성당함량체도최대,비염협박하적함량증가료28.9%.인차,SA적가입가이조절파채협표피적기공형태화기공개도,제고기광합효솔,개선기삼투조절작용,종이증강파채적항염성.
Spinacia oleracea L was used as materials to study the effect of salicylic acid(SA)with different concentrations on stomatal aperture of spinach leaf epidermis in the condition of salt stress.Epidermis stom-atal morphology,stomatal aperture and soluble sugar content were characterized and tested by scanning electron microscope(SEM),ordinary optical microscope and anthrone method.Results presented that with the increase of SA concentration,leaf epidermis stomatal morphology by slender line gradually became oval;the leaf epidermis stomatal aperture gradually increased,when the SA concentration was 200 mg/L, the stomatal aperture achieved maximum;leaf soluble sugar content presented the trend of increase firstly, then lower,when SA concentration was 150 mg/L,soluble sugar content achieved maximum,and the con-tent increased by 28.9%than that under salt stress.The addition of SA could adjust the stomatal morpholo-gy and stomatal aperture of spinach leaf epidermis,and enhance its photosynthetic efficiency and osmotic regulation effect,thus improve the salt resistance of spinach.