CAJ | 학술논문

通过云芝菌发酵得到的漆酶粗酶液经超滤浓缩后，以硫酸铵为盐析剂进行分级盐析沉淀，利用DEAE－Sepharose－F．F．柱层析对漆酶的发酵液进行分离纯化．SDS－PAGE电泳分析证明获得了电泳纯漆酶，其分子量约为64．4 kD．分离纯化过程中酶被纯化了22．80倍，酶活的回收率为36．32％，表明云芝菌产漆酶得到了很好的分离纯化．将漆酶液用于烟梗木质素的降解，实验结果表明，经此降解处理过的烟梗其内在品质得到了一定的提高：杂气和刺激性明显降低，木质气明显减弱，香气和余味均有改善．
통과운지균발효득도적칠매조매액경초려농축후，이류산안위염석제진행분급염석침정，이용DEAE－Sepharose－F．F．주층석대칠매적발효액진행분리순화．SDS－PAGE전영분석증명획득료전영순칠매，기분자량약위64．4 kD．분리순화과정중매피순화료22．80배，매활적회수솔위36．32％，표명운지균산칠매득도료흔호적분리순화．장칠매액용우연경목질소적강해，실험결과표명，경차강해처리과적연경기내재품질득도료일정적제고：잡기화자격성명현강저，목질기명현감약，향기화여미균유개선．
After the laccase crude enzyme which was obtained by fermenting Coriolus versicolor had been concentrated and hyperfiltrated,grading salting precipitation and DEAE-Sepharose-F.F.column chromatog-raphy had been taken to separate and purify the laccase fermented liquid with ammonium sulfate as the sal-ting-out agent.SDS-PAGE electrophoresis results showed that pure laccase could be obtained,the molecular weight was about 64.4 kD,enzyme was purified for 22.80 times by the purification process,the enzyme ac-tivity recovery rate was 36.32%.These proved that the laccase which was produced by Coriolus versicolor could be well purified.Using the enzyme solution in the experiment stem lignin degradation,the results showed that the intrinsic quality of the processed tobacco stems had been improved to some extent,miscel-laneous gases and irritating significantly reduced,wood gas also significantly reduced,aroma and aftertaste slightly improved.