CAJ | 학술논문

采用急性毒性试验方法研究异源铜盐对仿刺参（ Apostichopus japonicus）幼参的毒性影响，通过24 h、48 h、72 h和96 h的致死数量统计分析硫酸铜（ CuSO4）/氯化铜（ CuCl2）对幼参的半致死质量浓度（ LC50）和安全质量浓度（ SC）；利用石蜡切片显微定性观察不同铜盐对刺参幼参后肠的毒性情况。结果表明，CuSO4来源的Cu2+对幼参24 h、48 h和72 h的LC50均大于CuCl2来源的Cu2+对幼参24 h、48 h和72 h的LC50，而CuCl2对幼参96 h LC50大于CuSO4对幼参96 h LC50；异源铜盐在暴露前48 h内对刺参的毒性影响极其显著，随着暴露时间的延长其对刺参的毒性影响不显著；2种铜盐（以铜离子质量浓度为0.06 mg·L-1计）对幼参的消化道均有不同程度的腐蚀作用，导致结缔层与上皮层分离，CuCl2对刺参肠道的毒性（类似腐蚀作用）大于CuSO4的毒性；不同的阴离子对Cu2+的毒性有拮抗作用。
채용급성독성시험방법연구이원동염대방자삼（ Apostichopus japonicus）유삼적독성영향，통과24 h、48 h、72 h화96 h적치사수량통계분석류산동（ CuSO4）/록화동（ CuCl2）대유삼적반치사질량농도（ LC50）화안전질량농도（ SC）；이용석사절편현미정성관찰불동동염대자삼유삼후장적독성정황。결과표명，CuSO4래원적Cu2+대유삼24 h、48 h화72 h적LC50균대우CuCl2래원적Cu2+대유삼24 h、48 h화72 h적LC50，이CuCl2대유삼96 h LC50대우CuSO4대유삼96 h LC50；이원동염재폭로전48 h내대자삼적독성영향겁기현저，수착폭로시간적연장기대자삼적독성영향불현저；2충동염（이동리자질량농도위0.06 mg·L-1계）대유삼적소화도균유불동정도적부식작용，도치결체층여상피층분리，CuCl2대자삼장도적독성（유사부식작용）대우CuSO4적독성；불동적음리자대Cu2+적독성유길항작용。
The toxic effect of different copper salts on juvenile sea cucumbers was studied by acute toxicity test. LC50 and safety con-centration of two copper ions( CuSO4 and CuCl2 )were determined by statistical analysis of dead numbers of juvenile sea cucumbers ( Apostichopus japonicus)after treatments of 24 h,48 h,72 h and 96 h,respectively. The toxic effect of different copper salts on foregut of juvenile sea cucumbers were observed on paraffin section with microscope. The results show that LC50 s of CuSO4 on juvenile sea cucumbers after treatment of 24 h,48 h and 72 h were higher than that of CuCl2 ,while LC50 of CuCl2 was higher than that of Cu-SO4 after 96 h treatment. The difference of toxic effect of juvenile sea cucumbers exposed to the two copper ions within 48 h was very significant,but was not significant as the exposure time. Corrosive actions were observed in digestive tracts of juveniles treated with two kinds of salts( with concentration of 0. 06 mg·L-1 as copper ion),which resulted in connective tissue layer separating from the ep-ithelial layer. The toxicity( corrosive action)of CuCl2 on digestive tracts of sea cucumbers was greater than that of CuSO4 . Different anions of copper salts had antagonistic effect to copper toxicity.