CAJ | 학술논문

利用微电极植入技术，提出了一种新型的生物嗅觉传感系统，将多通道植入式微电极包埋于大鼠嗅球中，提取出嗅球神经元的气味响应信息。通过分析神经元响应活动从而进行气味检测与区分，发现该系统能够长期稳定地用于气味检测，且灵敏度达到10?10 mol/L。在单分子气味或自然气味检测中，该系统都具有很高的特异性。因此，该系统在实际气味检测(如肺癌呼出气体标志物检测、食品新鲜度检测、爆炸物搜索等)领域具有广泛的应用前景。
이용미전겁식입기술，제출료일충신형적생물후각전감계통，장다통도식입식미전겁포매우대서후구중，제취출후구신경원적기미향응신식。통과분석신경원향응활동종이진행기미검측여구분，발현해계통능구장기은정지용우기미검측，차령민도체도10?10 mol/L。재단분자기미혹자연기미검측중，해계통도구유흔고적특이성。인차，해계통재실제기미검측(여폐암호출기체표지물검측、식품신선도검측、폭작물수색등)영역구유엄범적응용전경。
Mammalian olfactory system can accurately identify odorants with high specificity and sensitivity. Taking advantage of multiple microelectrode implant technology, we developed a novel in vivo biosensing system for odor detection. The odorant information from conscious rats’ olfactory bulb (OB) is extracted by using microelectrode array. High-quality mitral/tufted (M/T) cell activity evoked by odorants could be obtained for at least three weeks. The responses of M/T cell carry sufficient information to discriminate presented odorants. In addition, we found the concentration detection limit of rat is below 10-10 mol/L for carvone. These results demonstrate that in vivo biosensing system has the characteristics of high sensitivity, continuous recording, and specificity, which presents a promising platform for specific trace odorant detection in many fields.