包头医学院学报
包頭醫學院學報
포두의학원학보
JOURNAL OF BAOTOU MEDICAL COLLEGE
2014年
6期
13-17
,共5页
杜晓%闫旭龙%陈梅%金刚%宋鸿雁%王丛%杨晓敏%王增帅%吴国霞
杜曉%閆旭龍%陳梅%金剛%宋鴻雁%王叢%楊曉敏%王增帥%吳國霞
두효%염욱룡%진매%금강%송홍안%왕총%양효민%왕증수%오국하
两肾一夹%高血压%糖尿病%大鼠模型
兩腎一夾%高血壓%糖尿病%大鼠模型
량신일협%고혈압%당뇨병%대서모형
Two -kidney,one clip%high blood pressure%diabetes mellitus%rat model
目的:建立肾血管性高血压合并2型糖尿病大鼠复合模型,通过检测不同时段的血压、血糖来观察此模型的稳定性。方法:雄性SD大鼠先采用两肾一夹法(2K1C )并饮用1.5%盐水建立高血压大鼠模型,在此模型成模的基础上喂养高糖、高脂饮食4周后经腹腔注射链尿佐菌素(35 mg/kg),然后继续予高糖、高脂饮食饲养至实验结束,检测不同时间点血压、空腹血糖、空腹胰岛素、血脂、血清C-反应蛋白,计算胰岛素敏感指数,并观察一般情况对所建模型进行评价。结果:高血压合并糖尿病组大鼠成模率70%,其血压、血糖值达到成模标准,至实验结束时较稳定,分别与假手术组、空白对照组比较差异有统计学意义( P <0.05)。结论:成功复制高血压合并2型糖尿病大鼠模型,该模型经济、操作简单、成模率高、稳定性好。
目的:建立腎血管性高血壓閤併2型糖尿病大鼠複閤模型,通過檢測不同時段的血壓、血糖來觀察此模型的穩定性。方法:雄性SD大鼠先採用兩腎一夾法(2K1C )併飲用1.5%鹽水建立高血壓大鼠模型,在此模型成模的基礎上餵養高糖、高脂飲食4週後經腹腔註射鏈尿佐菌素(35 mg/kg),然後繼續予高糖、高脂飲食飼養至實驗結束,檢測不同時間點血壓、空腹血糖、空腹胰島素、血脂、血清C-反應蛋白,計算胰島素敏感指數,併觀察一般情況對所建模型進行評價。結果:高血壓閤併糖尿病組大鼠成模率70%,其血壓、血糖值達到成模標準,至實驗結束時較穩定,分彆與假手術組、空白對照組比較差異有統計學意義( P <0.05)。結論:成功複製高血壓閤併2型糖尿病大鼠模型,該模型經濟、操作簡單、成模率高、穩定性好。
목적:건립신혈관성고혈압합병2형당뇨병대서복합모형,통과검측불동시단적혈압、혈당래관찰차모형적은정성。방법:웅성SD대서선채용량신일협법(2K1C )병음용1.5%염수건립고혈압대서모형,재차모형성모적기출상위양고당、고지음식4주후경복강주사련뇨좌균소(35 mg/kg),연후계속여고당、고지음식사양지실험결속,검측불동시간점혈압、공복혈당、공복이도소、혈지、혈청C-반응단백,계산이도소민감지수,병관찰일반정황대소건모형진행평개。결과:고혈압합병당뇨병조대서성모솔70%,기혈압、혈당치체도성모표준,지실험결속시교은정,분별여가수술조、공백대조조비교차이유통계학의의( P <0.05)。결론:성공복제고혈압합병2형당뇨병대서모형,해모형경제、조작간단、성모솔고、은정성호。
Objective :Establishing a compound model with renal vascular hypertension combined type 2 diabetic rats so as to observe its stability by monitoring the blood and pressure in different time periods .Methods :First ,we selected Male SD rats and adopted two kidney one clip method and drinking 1 .5% saline to establish hypertensive rat model ,on the basis of this model ,at the 4th week in high glucose ,and high fat feeding ,we injected abdominal cavity with strepto_zotocin (STZ 35mg/kg) .Second ,we continued to feed it with high -sugar high-fat diet until the experiment ended .Fi_nally ,we observed the changes of blood pressure ,fasting blood glucose(FBG) ,fasting insulin (FINS) ,lipids ,serum C- reactive protein (CRP) ,insulin sensitivity index (ISI) in different time points .Meanwhile ,we observed the model e_valuation in general situation .Results :About 70% of renal vascular hypertension combined type 2 diabetic rats were made into models ,all the succeed models had a stable blood pressure and blood glucose from its established to the end of the experiment .It has a statistically significant difference compared with the control group ,sham operation group(P <0 .05) .Conclusion :Successfully replicated hypertension combined with type 2 diabetic rat model ,which has the advantage of high economic value ,easy operation ,high modulus and good stability .