包头医学院学报
包頭醫學院學報
포두의학원학보
JOURNAL OF BAOTOU MEDICAL COLLEGE
2014年
6期
9-12
,共4页
骨性关节炎%5-氟尿嘧啶%玻璃酸钠%关节内注射%大鼠
骨性關節炎%5-氟尿嘧啶%玻璃痠鈉%關節內註射%大鼠
골성관절염%5-불뇨밀정%파리산납%관절내주사%대서
Osteoarthritis%5-Fluorouracil%Sodium hyaluronate%Intra-articular injection%Rats
目的：评价关节腔内注射5－氟尿嘧啶（Flurouracil ，5－FU）联合玻璃酸钠（sodium hyaluronate ，SH）对大鼠膝骨关节炎的疗效。方法：将36只Wistar大鼠制成骨关节炎模型后随机分为5－FU加SH组、SH组和对照组工厂组，分别给予5－FU联合S H、S H和生理盐水关节腔内注射，每周1次连续4次，最后一次治疗后1周处死实验动物，观察3组动物软骨和滑膜组织的基质金属蛋白酶（matrix metalloproteinases －1，MMP－1）免疫组化改变；对软骨进行Mankin＇s评分；比较3组动物血液中白介素－1（interleukin－1，IL －1）的浓度变化。结果：与SH组和对照组比较，5－Fu加SH组软骨病理切片显示软骨细胞凋亡减少，软骨基质结构完整，黏多糖含量多，滑膜细胞减少，滑膜下层的纤维增生减轻；血清中IL －1浓度明显降低（ P ＜0．01）；关节软骨中MMP－1表达明显减弱（ P ＜0．01）；Mankin＇s评分有明显改善（ P＜0．01）。结论：采用关节腔内5－FU加SH注射法治疗大鼠膝骨关节炎，能保护软骨基质，减轻滑膜增生，延缓软骨退行性变的进展。5－FU与SH联合用药可能成为治疗骨性关节炎的一种新的方法。
目的：評價關節腔內註射5－氟尿嘧啶（Flurouracil ，5－FU）聯閤玻璃痠鈉（sodium hyaluronate ，SH）對大鼠膝骨關節炎的療效。方法：將36隻Wistar大鼠製成骨關節炎模型後隨機分為5－FU加SH組、SH組和對照組工廠組，分彆給予5－FU聯閤S H、S H和生理鹽水關節腔內註射，每週1次連續4次，最後一次治療後1週處死實驗動物，觀察3組動物軟骨和滑膜組織的基質金屬蛋白酶（matrix metalloproteinases －1，MMP－1）免疫組化改變；對軟骨進行Mankin＇s評分；比較3組動物血液中白介素－1（interleukin－1，IL －1）的濃度變化。結果：與SH組和對照組比較，5－Fu加SH組軟骨病理切片顯示軟骨細胞凋亡減少，軟骨基質結構完整，黏多糖含量多，滑膜細胞減少，滑膜下層的纖維增生減輕；血清中IL －1濃度明顯降低（ P ＜0．01）；關節軟骨中MMP－1錶達明顯減弱（ P ＜0．01）；Mankin＇s評分有明顯改善（ P＜0．01）。結論：採用關節腔內5－FU加SH註射法治療大鼠膝骨關節炎，能保護軟骨基質，減輕滑膜增生，延緩軟骨退行性變的進展。5－FU與SH聯閤用藥可能成為治療骨性關節炎的一種新的方法。
목적：평개관절강내주사5－불뇨밀정（Flurouracil ，5－FU）연합파리산납（sodium hyaluronate ，SH）대대서슬골관절염적료효。방법：장36지Wistar대서제성골관절염모형후수궤분위5－FU가SH조、SH조화대조조공엄조，분별급여5－FU연합S H、S H화생리염수관절강내주사，매주1차련속4차，최후일차치료후1주처사실험동물，관찰3조동물연골화활막조직적기질금속단백매（matrix metalloproteinases －1，MMP－1）면역조화개변；대연골진행Mankin＇s평분；비교3조동물혈액중백개소－1（interleukin－1，IL －1）적농도변화。결과：여SH조화대조조비교，5－Fu가SH조연골병리절편현시연골세포조망감소，연골기질결구완정，점다당함량다，활막세포감소，활막하층적섬유증생감경；혈청중IL －1농도명현강저（ P ＜0．01）；관절연골중MMP－1표체명현감약（ P ＜0．01）；Mankin＇s평분유명현개선（ P＜0．01）。결론：채용관절강내5－FU가SH주사법치료대서슬골관절염，능보호연골기질，감경활막증생，연완연골퇴행성변적진전。5－FU여SH연합용약가능성위치료골성관절염적일충신적방법。
Objective :To evaluate the curative effect of intra -articular injection of Flurouracil (5 -FU ) combined with sodium hyaluronate (SH) in the treatment of knee osteoarthritis in rats .Methods :Thirty -six osteoarthritis (OA) model rats were randomly divided into the 5-FU plus SH group ,the SH group and the control group .The three groups were treated with intra -articular injection of 5-FU combined with SH ,SH and physiologic saline ,respectively ,once a week ,for 4 weeks .The model rats were put to death one week after the last injection .The immunochemical changes in matrix metalloproteinases -1 (MMP-1) of cartilage and synovium were observed in the three groups .Mankin's grading system was used for rating of the cartilage .The levels of interleukin -1(IL -1β) in the blood were compared among the three groups .Results :Compared with the SH group and the control group ,the 5-FU with SH group showed reduced apoptosis of chondrocytes and they also kept a more integrated structure of matrix and contained more glycosaminoglycan . The synoviocytes were reduced ;the fibrous hyperplasia in synovial underlayer was inhibited ;The level of IL -1βin the serum dropped( P < 0 .01);the expression of MMP -1 in the cartilage was significantly decreased ( P < 0 .01);the Mankin's score was significantly decreased ( P <0 .01) .Conclusion :Intra -articular injection of 5 -FU combined with SH can relieve OA ,protect the matrix of cartilage ,decrease the synoviocyttes ,inhibit fibrous hyperplasia and delay the cataplasia of the articular cartilage .Thus ,it can be a new way to treat osteoarthritis .
