CAJ | 학술논문

目的观察不同剂量HS6101对环磷酰胺（cyclophosphamide，CTX）损伤小鼠造血功能恢复的影响。方法正常ICR小鼠连续3 d腹腔注射CTX 100 mg/（kg·d）制备化疗药损伤模型，3组动物于首次CTX前1 d，每只分别皮下注射HS61010、9和27μg，每组动物数分别为20只，观察各组小鼠外周血细胞计数、第4和9天骨髓造血祖细胞集落数和骨髓病理组织学变化。结果不同剂量HS6101均可明显升高CTX化疗小鼠外周血白细胞和中性粒细胞数（P<0.05），增加骨髓各系造血祖细胞集落生成，刺激化疗损伤后骨髓细胞增殖。其中HS610127μg给药组效果更佳。结论 HS610127μg可明显促进CTX化疗ICR小鼠造血功能的恢复。
목적관찰불동제량HS6101대배린선알（cyclophosphamide，CTX）손상소서조혈공능회복적영향。방법정상ICR소서련속3 d복강주사CTX 100 mg/（kg·d）제비화료약손상모형，3조동물우수차CTX전1 d，매지분별피하주사HS61010、9화27μg，매조동물수분별위20지，관찰각조소서외주혈세포계수、제4화9천골수조혈조세포집락수화골수병리조직학변화。결과불동제량HS6101균가명현승고CTX화료소서외주혈백세포화중성립세포수（P<0.05），증가골수각계조혈조세포집락생성，자격화료손상후골수세포증식。기중HS610127μg급약조효과경가。결론 HS610127μg가명현촉진CTX화료ICR소서조혈공능적회복。
Objective To observe the effect of different doses of HS6101 on the recovery of hematopoietic injury in ICR mice treated with cyclophosphamide (CTX). Methods Normal ICR mice were intraperitoneally injected with CTX at 100 mg/kg once a day for 3 consecutive days,and the mouse model of chemotherapy-induced hematopoietic injury was established. Three groups of mice (with 20 per group),were respectively injected with HS6101 at 0,9 or 27 μg subcutaneously at one hour before the first administration of CTX. The peripheral blood cell counts of the mice were observed once every 2 days. Hematopoietic progenitor cell colony counting and histopathological assessment of bone marrow cells were evaluated at 4 d and 9 d after the first administration of CTX. Results In ICR mice after chemotherapy with CTX,all doses of HS6101 significantly increased peripheral leukocytes and neutrophils (P<0.05),elevated the number of multilineage hematopoietic progenitor cell colonies of bone marrow,and stimulated the proliferation of bone marrow cells after CTX injury. Mice receiving 27 μg HS6101 were better than those of the other two groups. Conclusion HS6101 at 27 μg could significantly promote the recovery of hematopoiesis in ICR mice treated with CTX chemotherapy.