CAJ | 학술논문

目的：对表达巴斯德毕赤酵母的发酵条件进行优化，确定最佳的发酵控制条件，以获得重组textilinin‐1（Q8008）最高表达量。方法通过多因素正交实验确定 Q8008发酵培养的最适发酵条件。结果表达温度在22℃，pH 值为7．0，加入甲醇量为5 g／L 时为最优发酵条件，诱导表达时间为84～108 h。结论优化巴斯德毕赤酵母的发酵条件，可提高 Q8008表达量，为开发抗纤溶酶止血药应用于临床具有重要意义。
목적：대표체파사덕필적효모적발효조건진행우화，학정최가적발효공제조건，이획득중조textilinin‐1（Q8008）최고표체량。방법통과다인소정교실험학정 Q8008발효배양적최괄발효조건。결과표체온도재22℃，pH 치위7．0，가입갑순량위5 g／L 시위최우발효조건，유도표체시간위84～108 h。결론우화파사덕필적효모적발효조건，가제고 Q8008표체량，위개발항섬용매지혈약응용우림상구유중요의의。
Objective In order to provide theoretica1 basis for the industrial production of Q8008 .The fermenta‐tion conditions of the recombinant pichia pastoris strain X‐33/pPICZαA‐Txln‐1 in shake flask were studied . Methods The optimum conditions are selected by orthogonal design . Results The optimum temperature is 22℃ ,pH is 7.0 ,methanol concentration is 5 g/L ,time is 84~108 h .The activity of Q8008 was reached to 30 PIU/ml . Conclusion Q8008 can be developing as inhibit plasmin haemostat ,hope to take the place of clinic applied apro tinin .