CAJ | 학술논문

建立了一种基于微生物法检测婴幼儿配方奶粉中生物素含量的快速检测方法。采用该方法检测生物素时其标准曲线相关系数为0.9997，平行测试的RSD为2.89%-4.92%，加标实验回收率在90.3%-97.3%之间。与传统国标方法相比，该方法操作简单，优化了样品前处理过程，缩短了实验周期，提高了效率。与试剂盒法相比，该方法的准确度和重现性良好，且经济实用，适合在实验室推广应用。
건립료일충기우미생물법검측영유인배방내분중생물소함량적쾌속검측방법。채용해방법검측생물소시기표준곡선상관계수위0.9997，평행측시적RSD위2.89%-4.92%，가표실험회수솔재90.3%-97.3%지간。여전통국표방법상비，해방법조작간단，우화료양품전처리과정，축단료실험주기，제고료효솔。여시제합법상비，해방법적준학도화중현성량호，차경제실용，괄합재실험실추엄응용。
This paper established a rapid microbiological detection based method of biotin in infant formula milk powder. By using this method, correlation coeffi cient of the standard curve was 0.9997, RSD of parallel tests was between 2.89% and 4.92%, and recovery rate of standard addition experiment was between 90.3% and 97.3%. Compared with traditional GB method, the new method optimized the pre-treatment process of sample, which was easy to operate with shorter experimental period and higher effi ciency. Compared with kit method, the new method featured with higher accuracy, better reproducibility, and more economical and practical, which was suitable for popularization and application in the laboratory.