CAJ | 학술논문

2012年至2013年在甘肃省各当归主产区发现的一种疑似当归炭疽病的病害，该病害主要危害植株茎秆。发病初期先在植株外部茎秆上出现浅褐色病斑，随后病斑逐渐扩大，形成深褐色长条形病斑，叶片变黄枯死，茎秆从外向内逐渐干枯死亡，后期在病斑上出现黑色小颗粒（分生孢子盘），最后整株枯死。通过病菌形态学鉴定、ITS 基因序列分析以及致病性测定，已确定该病害是由束状炭疽菌危害引起的当归炭疽病。本研究系首次报道。经过室内盆栽接种试验表明，针刺、灌根和喷雾接种均能发病，以针刺发病最重，其次为灌根处理，喷雾接种最轻，表明病菌通过伤口、根部以及地上部分均可侵入；此病一般在6月中、下旬开始发生，8月下旬到9月上旬达到发病高峰，田间发病率、病情指数与气温和相对湿度存在极显著的正相关，即温度高、湿度大有利于病害发生，且湿度的影响大于温度影响。本研究为当归炭疽病的防治提供了依据。
2012년지2013년재감숙성각당귀주산구발현적일충의사당귀탄저병적병해，해병해주요위해식주경간。발병초기선재식주외부경간상출현천갈색병반，수후병반축점확대，형성심갈색장조형병반，협편변황고사，경간종외향내축점간고사망，후기재병반상출현흑색소과립（분생포자반），최후정주고사。통과병균형태학감정、ITS 기인서렬분석이급치병성측정，이학정해병해시유속상탄저균위해인기적당귀탄저병。본연구계수차보도。경과실내분재접충시험표명，침자、관근화분무접충균능발병，이침자발병최중，기차위관근처리，분무접충최경，표명병균통과상구、근부이급지상부분균가침입；차병일반재6월중、하순개시발생，8월하순도9월상순체도발병고봉，전간발병솔、병정지수여기온화상대습도존재겁현저적정상관，즉온도고、습도대유리우병해발생，차습도적영향대우온도영향。본연구위당귀탄저병적방치제공료의거。
A suspected anthracnose disease on Angelica stems was found in the main producing areas in Gansu province in 2012 and 2013.The major damage of this disease is to the plant stem.In the initial stages of the disease,many light brown spots appear on stems,and then expand to dark brown rectangular lesions,leaf chlorosis and wilting.Stems gradually dry up from outside to inside,abundant black acervuli appear on the spots,and then the plant dies.Through pathogenicity testing,morphology and ITS sequence analysis we de-termined that the pathogen of Angelica anthrax is Colletotrichum dematium .As far as we know,this study is the first report for this disease.An inoculation test using potted plants indoors shows that puncturing,root-ir-rigation and spraying with conidial suspension all can cause disease.Puncturing caused the most severe symp-toms.Spraying with suspension induced the lightest symptoms.C.dematium can infect Angelica through wounds,roots and aerial parts.This disease first appears in mid or late June,and incidence peaks in late Au-gust or early September.Field temperature and relative humidity are significantly positively correlated with disease incidence and severity,suggesting that high temperature and high humidity promote progress of the in-fection.The impact of humidity on disease development is greater than temperature.This study provides a ba-sis for developing prevention and control methods for Angelica anthrax.