草业学报
草業學報
초업학보
PRATACULTURAL SCIENCE
2014年
6期
189-197
,共9页
刘会杰%李胜%马绍英%张品南%时振振%杨晓明
劉會傑%李勝%馬紹英%張品南%時振振%楊曉明
류회걸%리성%마소영%장품남%시진진%양효명
豌豆%H2 O2%初生根%外源 Ca2 +%抗氧化物酶
豌豆%H2 O2%初生根%外源 Ca2 +%抗氧化物酶
완두%H2 O2%초생근%외원 Ca2 +%항양화물매
pea (Pisum sativum )%H2 O2%primary root%exogenous Ca2 +%antioxidase
以豌豆品种“陇豌一号”为材料,通过对豌豆种子萌发初期初生根生理特性的研究,探索提高豌豆初生根外源H2 O2胁迫条件下抗性能力的途径。运用生理生化方法,测定 H2 O2胁迫下豌豆初生根在外源 Ca2+处理后的弯曲率和根系活力,并对豌豆初生根内的丙二醛(MDA)含量、相对膜透性、超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性进行测定。结果显示,80 mmol/L 的 H2 O2处理下的豌豆初生根正常生长受到显著抑制,但是经过 Ca2+处理后,根系生长抑制作用得到缓解,根系活力得以恢复。外施10 mmol/L Ca2+初生根 MDA 值较 CK1降低了37.32%,并显著地提高了 POD,SOD,CAT 和 APX 的活性,其值分别为51.946 U/(mg·min),865.174 U/g FW,1.9739 mmol/(L·g·min)和2.569μmol/(L·g·min)。总之,外源施加 Ca2+能够有效降低 H2 O2造成的氧化胁迫,缓解对初生根细胞膜的伤害,降低质膜透性,增强初生根系抗氧化酶活性,达到抵抗逆境胁迫的目的。
以豌豆品種“隴豌一號”為材料,通過對豌豆種子萌髮初期初生根生理特性的研究,探索提高豌豆初生根外源H2 O2脅迫條件下抗性能力的途徑。運用生理生化方法,測定 H2 O2脅迫下豌豆初生根在外源 Ca2+處理後的彎麯率和根繫活力,併對豌豆初生根內的丙二醛(MDA)含量、相對膜透性、超氧化物歧化酶(SOD)、過氧化物酶(POD)、過氧化氫酶(CAT)和抗壞血痠過氧化物酶(APX)活性進行測定。結果顯示,80 mmol/L 的 H2 O2處理下的豌豆初生根正常生長受到顯著抑製,但是經過 Ca2+處理後,根繫生長抑製作用得到緩解,根繫活力得以恢複。外施10 mmol/L Ca2+初生根 MDA 值較 CK1降低瞭37.32%,併顯著地提高瞭 POD,SOD,CAT 和 APX 的活性,其值分彆為51.946 U/(mg·min),865.174 U/g FW,1.9739 mmol/(L·g·min)和2.569μmol/(L·g·min)。總之,外源施加 Ca2+能夠有效降低 H2 O2造成的氧化脅迫,緩解對初生根細胞膜的傷害,降低質膜透性,增彊初生根繫抗氧化酶活性,達到牴抗逆境脅迫的目的。
이완두품충“롱완일호”위재료,통과대완두충자맹발초기초생근생리특성적연구,탐색제고완두초생근외원H2 O2협박조건하항성능력적도경。운용생리생화방법,측정 H2 O2협박하완두초생근재외원 Ca2+처리후적만곡솔화근계활력,병대완두초생근내적병이철(MDA)함량、상대막투성、초양화물기화매(SOD)、과양화물매(POD)、과양화경매(CAT)화항배혈산과양화물매(APX)활성진행측정。결과현시,80 mmol/L 적 H2 O2처리하적완두초생근정상생장수도현저억제,단시경과 Ca2+처리후,근계생장억제작용득도완해,근계활력득이회복。외시10 mmol/L Ca2+초생근 MDA 치교 CK1강저료37.32%,병현저지제고료 POD,SOD,CAT 화 APX 적활성,기치분별위51.946 U/(mg·min),865.174 U/g FW,1.9739 mmol/(L·g·min)화2.569μmol/(L·g·min)。총지,외원시가 Ca2+능구유효강저 H2 O2조성적양화협박,완해대초생근세포막적상해,강저질막투성,증강초생근계항양화매활성,체도저항역경협박적목적。
In order to explore methodology for improving the resistance of pea primary roots to H2 O2 stress, physiological characteristics of pea primary roots were studied using a pea variety “Longwan 1 ”treated with Ca2 + under H2 O2 stress.Several physiological indexes like the curvature rate and root activity of pea primary roots were measured using physiological and biochemical methods.Some other indexes like the relative mem-brane permeability of primary roots,the concentration of malondialdehyde (MDA)in primary roots,the activi-ties of superoxide dismutase (SOD),peroxidase (POD),catalase (CAT)and ascorbate peroxidase (APX)en-zymes were also measured.The normal growth of pea primary roots under 80 mmol/L H2 O2 stress was obvi-ously inhibited.However,the inhibitory effect on root growth was reduced and the root activity was restored after treating with Ca2 + .The MDA content decreased 37.32% compared to controls after treatment with 10 mmol/L exogenous Ca2 + .The activities of four enzymes including POD,SOD,CAT and APX were enhanced and reached 51.946 U/(mg·min),865.174 U/g FW,1.9739 mmol/(L·g·min)and 2.569 μmol/(L·g· min),respectively.In summary,Ca2 + (10 mmol/L)significantly alleviated the damage to pea primary root cell membranes caused by H2 O2 stress,reduced membrane permeability and promoted the antioxidant capacity of pea primary roots for stress resistance.