CAJ | 학술논문

采用斑马鱼胚胎发育技术和传统毒理学方法,研究了氟吡菌胺对斑马鱼胚胎、成鱼及仔鱼的毒性效应.结果发现,氟吡菌胺对3个阶段斑马鱼均具有致死能力,对仔鱼LC50(48h)值为0.204mg/L,成鱼LC50(96h)为0.286mg/L,幼鱼LC50(96h)为1.489mg/L.研究表明,0.0596mg/L以上浓度的氟吡菌胺对斑马鱼胚胎均有一定程度的致死效应,高浓度处理组胚胎出现心包囊肿、卵黄囊不吸收、黑色素沉积少及鱼体弯曲等症状；氟吡菌胺对斑马鱼成鱼进行14d暴露后,处理组成鱼体重下降,产卵量减少,有效卵量降低；0.0298mg/L处理组144h子代仔鱼存活率虽然比较高,但存活鱼大部分出现心包囊肿、体弯曲等畸形.上述结果说明,水体中残留的氟吡菌胺对于斑马鱼各生命阶段的生长发育均具有潜在的危害.
채용반마어배태발육기술화전통독이학방법,연구료불필균알대반마어배태、성어급자어적독성효응.결과발현,불필균알대3개계단반마어균구유치사능력,대자어LC50(48h)치위0.204mg/L,성어LC50(96h)위0.286mg/L,유어LC50(96h)위1.489mg/L.연구표명,0.0596mg/L이상농도적불필균알대반마어배태균유일정정도적치사효응,고농도처리조배태출현심포낭종、란황낭불흡수、흑색소침적소급어체만곡등증상；불필균알대반마어성어진행14d폭로후,처리조성어체중하강,산란량감소,유효란량강저；0.0298mg/L처리조144h자대자어존활솔수연비교고,단존활어대부분출현심포낭종、체만곡등기형.상술결과설명,수체중잔류적불필균알대우반마어각생명계단적생장발육균구유잠재적위해.
The zebrafish embryos technology and traditional toxicology methods were used to determined the toxicity of fluopicolide to zebrafish embryos、adult fish and larvae. Fluopicolide had lethal capabilities to the three stages of zebrafish with the LC50(48h) values to larvae and LC50(96h) to adult fish and juvenile was 0.204mg/L, 0.286mg/L and 1.489mg/L. 0.0596mg/L or more concentrations fluopicolide have a certain degree of lethal effect to zebrafish embryos, and the embryos which were treated with high concentrations displayed many of the symptoms, such as pericardial cyst, yolk sac does not absorb, less melanin deposition and fish bending. After 14days exposure with fluopicolide, the adult zebrafishs expressed the following symptoms:weight loss, reduction in fecundity, the amount of effective eggs were reduced;After treated by 0.0298mg/L fluopicolide, though 144h offspring larval’s survival relatively high, but most surviving fish has still emerged as deformity: pericardial edema and abnormal body bending, etc. Fluopicolide residue in water have the potential hazards to the growth and development of each life stage of zebrafish.